MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1249__2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues) |
| Timestamp | : | 23 Jun 2025 at 17:08:04 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,602 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RNE_ECOLI | 44 | Ribonuclease E OS=Escherichia coli (strain K12) GN=rne PE=1 SV=6 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LPXA_ECOLI | 42 | Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase OS=Escherichia coli (strain K12) GN=lpxA PE=1 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K1C10_HUMAN | 33 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 13.1 | K1C10_HUMAN |
33 | 59020 | 5 (2) | 5 (2) | 0.11 | |
| Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 | |||||||
5 peptide matches (5 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2268 |
404.2054 | 806.3962 | 806.3923 | 4.95 | 0 | 30 | 0.004 | 1Score > 19 indicates identity |
U | R.LAADDFR.L | |
| 2621 |
355.5425 | 1063.6057 | 1063.6026 | 2.92 | 1 | 5 | 0.45 | 1Score > 14 indicates identity |
U | R.LASYLDKVR.A | |
| 2672 |
553.7692 | 1105.5238 | 1105.5186 | 4.75 | 0 | 16 | 0.11 | 1Score > 19 indicates identity |
U | K.VTMQNLNDR.L + Oxidation (M) | |
| 2812 |
412.2325 | 1233.6757 | 1233.6717 | 3.23 | 1 | 9 | 0.28 | 1Score > 16 indicates identity |
U | R.LKYENEVALR.Q | |
| 3316 |
498.5848 | 1492.7326 | 1492.7270 | 3.75 | 1 | 22 | 0.032 | 1Score > 19 indicates identity |
U | R.SQYEQLAEQNRK.D |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| K1C15_SHEEP |
30 | 48740 | 13.1 | ||
| Keratin, type I cytoskeletal 15 OS=Ovis aries GN=KRT15 PE=2 SV=1 | |||||
| 8 samesets of K1C15_SHEEP | |||||
| KRT38_HUMAN |
30 | 52044 | |||
| Keratin, type I cuticular Ha8 OS=Homo sapiens GN=KRT38 PE=2 SV=3 | |||||
| K1H1_HUMAN |
30 | 48633 | |||
| Keratin, type I cuticular Ha1 OS=Homo sapiens GN=KRT31 PE=2 SV=3 | |||||
| K1H2_HUMAN |
30 | 51793 | |||
| Keratin, type I cuticular Ha2 OS=Homo sapiens GN=KRT32 PE=1 SV=3 | |||||
| K1M1_SHEEP |
30 | 48070 | |||
| Keratin, type I microfibrillar 48 kDa, component 8C-1 OS=Ovis aries PE=1 SV=2 | |||||
| KT33B_HUMAN |
30 | 47325 | |||
| Keratin, type I cuticular Ha3-II OS=Homo sapiens GN=KRT33B PE=2 SV=3 | |||||
| KRT35_HUMAN |
30 | 51640 | |||
| Keratin, type I cuticular Ha5 OS=Homo sapiens GN=KRT35 PE=2 SV=5 | |||||
| KRT36_HUMAN |
30 | 53354 | |||
| Keratin, type I cuticular Ha6 OS=Homo sapiens GN=KRT36 PE=1 SV=1 | |||||
| KRT37_HUMAN |
30 | 51084 | |||
| Keratin, type I cuticular Ha7 OS=Homo sapiens GN=KRT37 PE=2 SV=3 | |||||
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NARQ_ECOLI | 33 | description |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPB_ECOLI | 31 | Anaerobic glycerol-3-phosphate dehydrogenase subunit B OS=Escherichia coli (strain K12) GN=glpB PE=1 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL7_ECOLI | 31 | 50S ribosomal protein L7/L12 OS=Escherichia coli (strain K12) GN=rplL PE=1 SV=2 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OPPF_ECOLI | 30 | description |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YNJD_ECOLI | 29 | Uncharacterized ABC transporter ATP-binding protein YnjD OS=Escherichia coli (strain K12) GN=ynjD PE=3 SV=2 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RECB_ECOLI | 28 | RecBCD enzyme subunit RecB OS=Escherichia coli (strain K12) GN=recB PE=1 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YBJK_ECOLI | 26 | description |
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