MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:08:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 44)

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-1

Accession Score Description
1 YHBW_ECOLI 340 Uncharacterized protein YhbW OS=Escherichia coli (strain K12) GN=yhbW PE=4 SV=1
Score Mass Matches Sequences emPAI
1.1 YHBW_ECOLI 340 37163 15 (13) 10 (9) 1.78
Uncharacterized protein YhbW OS=Escherichia coli (strain K12) GN=yhbW PE=4 SV=1

-15 peptide matches (14 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2222   372.2249 742.4352 742.4337 2.04 0 36 0.0011 +1Score > 19 indicates identity U R.IDLGLGR.A
2359   453.7450 905.4754 905.4719 3.93 0 20 0.049 +1Score > 19 indicates identity U R.SNFKPSAR.L
2381 -1 462.2758 922.5370 922.5348 2.40 0 32 0.00062 +1Score > 13 indicates identity U R.HGLQSILR.E
2382   462.2768 922.5390 922.5348 4.57 0 (32) 0.00065 +1Score > 13 indicates identity U R.HGLQSILR.E
2834   623.3157 1244.6168 1244.6149 1.54 0 56 1.1e-005 +1Score > 19 indicates identity U R.EAFSHSLDLAR.L
2835   415.8797 1244.6173 1244.6149 1.88 0 55 1.4e-005 +1Score > 19 indicates identity U R.EAFSHSLDLAR.L
2962   435.5300 1303.5682 1303.5615 5.09 0 45 5.6e-005 +1Score > 15 indicates identity U R.HMSGDIDNFPR.D + Oxidation (M)
2963   652.7914 1303.5682 1303.5615 5.15 0 28 0.0026 +1Score > 15 indicates identity U R.HMSGDIDNFPR.D + Oxidation (M)
3250   718.3497 1434.6848 1434.6779 4.81 0 77 8.4e-008 +1Score > 19 indicates identity U R.DVAELVDWFDAR.D
3440   416.2164 1660.8365 1660.8322 2.61 0 10 0.5 +1Score > 19 indicates identity U R.DPNPHVRPVPGYGEK.I
3441   554.6201 1660.8385 1660.8322 3.80 0 11 0.38 +1Score > 20 indicates identity U R.DPNPHVRPVPGYGEK.I
3743   616.3166 1845.9280 1845.9182 5.28 1 52 3.6e-005 +1Score > 20 indicates identity U R.LHSFELAMDVKEELLG.- + Oxidation (M)
3894   653.3618 1957.0636 1957.0520 5.90 0 26 0.0054 +1Score > 16 indicates identity U K.TIAFSLLDLAPIPEGSSAR.E
4128   736.0034 2204.9884 2204.9644 10.9 0 47 5.5e-005 +1Score > 17 indicates identity U R.ETDADEIMVNGQIFDHQAR.L + Deamidated (NQ); Oxidation (M)
4129   736.3309 2205.9709 2205.9484 10.2 0 37 0.00038 +1Score > 15 indicates identity U R.ETDADEIMVNGQIFDHQAR.L + 2 Deamidated (NQ); Oxidation (M)

+2

Accession Score Description
1 Q9EX54_STRCO 282 Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1

+3

Accession Score Description
1 CH60_ECOLI 258 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2

+4

Accession Score Description
1 TRYP_PIG 176 Trypsin OS=Sus scrofa PE=1 SV=1

+5

Accession Score Description
1 CRP_ECOLI 142 cAMP-activated global transcriptional regulator CRP OS=Escherichia coli (strain K12) GN=crp PE=1 SV=1

+6

Accession Score Description
1 K2C1_HUMAN 119 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6

+7

Accession Score Description
1 EFTU1_ECOLI 103 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1

+8

Accession Score Description
1 RL4_ECOLI 74 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1

+9

Accession Score Description
1 ATDA_ECOLI 63 Spermidine N(1)-acetyltransferase OS=Escherichia coli (strain K12) GN=speG PE=1 SV=2

+10

Accession Score Description
1 ISPA_ECOLI 61 Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1
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