MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
296 312.1323 311.1250
297 312.1326 311.1253
298 313.0217 312.0144
299 313.0601 312.0528
300 313.0608 312.0535
301 313.0608 312.0535
302 313.0609 312.0536
303 313.0905 312.0832
304 313.0967 312.0894
305 313.0971 312.0898
306 313.0979 312.0906
307 313.1271 312.1198
308 313.1275 312.1202
309 313.1276 312.1203
310 313.1284 312.1211
311 313.1288 312.1215
312 313.1291 312.1218
313 313.1632 312.1559
314 313.1635 312.1562
315 313.1635 312.1562
316 313.1638 312.1565
317 313.1640 312.1567
318 313.1642 312.1569
319 313.1645 312.1572
320 313.1646 312.1573
321 313.1647 312.1574
322 313.1653 312.1580
323 313.1828 312.1755
324 313.1834 312.1761
325 313.1835 312.1762
326 313.1835 312.1762
327 314.0466 313.0393
328 314.0467 313.0394
329 314.1311 313.1238
330 314.1973 313.1900
331 314.1976 313.1903
332 314.1982 313.1909
333 315.0399 314.0326
334 315.0753 314.0680
335 315.0756 314.0683
336 315.0756 314.0683
337 315.0758 314.0685
338 315.0765 314.0692
339 315.1135 314.1062
340 315.1145 314.1072
341 315.1413 314.1340
342 315.1425 314.1352
343 315.1428 314.1355
344 315.1431 314.1358
345 315.1434 314.1361
346 315.1586 314.1513
347 315.1601 314.1528
348 315.1790 314.1717
349 315.1793 314.1720
350 316.0255 315.0182
351 316.1753 315.1680
352 316.1990 315.1917
353 316.2130 315.2057
354 316.2139 315.2066
355 317.0548 316.0475
356 317.0916 316.0843
357 317.0988 316.0915
358 317.0998 316.0925
359 317.1000 316.0927
360 317.1001 316.0928
361 317.1003 316.0930
362 317.1007 316.0934
363 317.1015 316.0942
364 317.1291 316.1218
365 317.1367 316.1294
366 317.1372 316.1299
367 317.1378 316.1305
368 317.1378 316.1305
369 317.1743 316.1670
370 317.1745 316.1672
371 317.1745 316.1672
372 317.1747 316.1674
373 317.1748 316.1675
374 317.1764 316.1691
375 317.1943 316.1870
376 317.1948 316.1875
377 317.1954 316.1881
378 318.0428 317.0355
379 318.1922 317.1849
380 318.1922 317.1849
381 318.1926 317.1853
382 318.1927 317.1854
383 318.8783 317.8710
384 318.8785 317.8712
385 319.0406 318.0333
386 319.0415 318.0342
387 319.0495 318.0422
388 319.0800 318.0727
389 319.1152 318.1079
390 319.1157 318.1084
391 319.1161 318.1088
392 319.1161 318.1088
393 319.1163 318.1090
394 319.1163 318.1090
395 319.1165 318.1092
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