MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
196 309.1317 308.1244
197 309.1317 308.1244
198 309.1317 308.1244
199 309.1317 308.1244
200 309.1318 308.1245
201 309.1319 308.1246
202 309.1321 308.1248
203 309.1322 308.1249
204 309.1322 308.1249
205 309.1323 308.1250
206 309.1324 308.1251
207 309.1324 308.1251
208 309.1324 308.1251
209 309.1324 308.1251
210 309.1324 308.1251
211 309.1325 308.1252
212 309.1325 308.1252
213 309.1326 308.1253
214 309.1327 308.1254
215 309.1327 308.1254
216 309.1677 308.1604
217 309.1681 308.1608
218 309.1682 308.1609
219 309.1682 308.1609
220 309.1683 308.1610
221 309.1684 308.1611
222 309.1685 308.1612
223 309.1685 308.1612
224 309.1685 308.1612
225 309.1686 308.1613
226 309.1686 308.1613
227 309.1686 308.1613
228 309.1688 308.1615
229 309.1692 308.1619
230 309.1692 308.1619
231 309.1693 308.1620
232 309.1693 308.1620
233 309.1693 308.1620
234 309.1693 308.1620
235 309.1695 308.1622
236 309.1695 308.1622
237 309.1695 308.1622
238 309.1701 308.1628
239 309.1883 308.1810
240 309.1884 308.1811
241 309.1884 308.1811
242 309.1886 308.1813
243 309.2057 308.1984
244 309.2057 308.1984
245 309.2063 308.1990
246 309.2064 308.1991
247 309.2065 308.1992
248 309.2066 308.1993
249 309.4840 308.4767
250 310.0155 309.0082
251 311.0450 310.0377
252 311.0810 310.0737
253 311.0816 310.0743
254 311.0816 310.0743
255 311.0816 310.0743
256 311.0831 310.0758
257 311.0900 310.0827
258 311.0907 310.0834
259 311.0908 310.0835
260 311.0999 310.0926
261 311.1103 310.1030
262 311.1109 310.1036
263 311.1109 310.1036
264 311.1110 310.1037
265 311.1113 310.1040
266 311.1113 310.1040
267 311.1115 310.1042
268 311.1116 310.1043
269 311.1116 310.1043
270 311.1117 310.1044
271 311.1473 310.1400
272 311.1475 310.1402
273 311.1477 310.1404
274 311.1477 310.1404
275 311.1479 310.1406
276 311.1479 310.1406
277 311.1479 310.1406
278 311.1481 310.1408
279 311.1481 310.1408
280 311.1482 310.1409
281 311.1483 310.1410
282 311.1484 310.1411
283 311.1484 310.1411
284 311.1485 310.1412
285 311.1485 310.1412
286 311.1485 310.1412
287 311.1659 310.1586
288 311.1662 310.1589
289 311.1664 310.1591
290 311.1665 310.1592
291 311.1842 310.1769
292 311.1843 310.1770
293 311.1850 310.1777
294 311.2233 310.2160
295 311.2235 310.2162
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