MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
96 305.1373 304.1300
97 305.1374 304.1301
98 305.1376 304.1303
99 305.1376 304.1303
100 305.1376 304.1303
101 305.1377 304.1304
102 305.1377 304.1304
103 305.1377 304.1304
104 305.1378 304.1305
105 305.1378 304.1305
106 305.1378 304.1305
107 305.1380 304.1307
108 305.1381 304.1308
109 305.1582 304.1509
110 305.1733 304.1660
111 305.1735 304.1662
112 305.1736 304.1663
113 305.1737 304.1664
114 305.1738 304.1665
115 305.1739 304.1666
116 305.1740 304.1667
117 305.1740 304.1667
118 305.1744 304.1671
119 305.1744 304.1671
120 305.1744 304.1671
121 305.1745 304.1672
122 305.1746 304.1673
123 305.1746 304.1673
124 305.1747 304.1674
125 305.1747 304.1674
126 305.2109 304.2036
127 305.9593 304.9520
128 306.1566 305.1493
129 306.1604 305.1531
130 306.1925 305.1852
131 307.0100 306.0027
132 307.0199 306.0126
133 307.0781 306.0708
134 307.0785 306.0712
135 307.0786 306.0713
136 307.0790 306.0717
137 307.0969 306.0896
138 307.1135 306.1062
139 307.1159 306.1086
140 307.1160 306.1087
141 307.1160 306.1087
142 307.1161 306.1088
143 307.1161 306.1088
144 307.1161 306.1088
145 307.1165 306.1092
146 307.1166 306.1093
147 307.1167 306.1094
148 307.1167 306.1094
149 307.1167 306.1094
150 307.1169 306.1096
151 307.1170 306.1097
152 307.1172 306.1099
153 307.1524 306.1451
154 307.1525 306.1452
155 307.1526 306.1453
156 307.1526 306.1453
157 307.1528 306.1455
158 307.1529 306.1456
159 307.1529 306.1456
160 307.1529 306.1456
161 307.1529 306.1456
162 307.1530 306.1457
163 307.1532 306.1459
164 307.1532 306.1459
165 307.1533 306.1460
166 307.1534 306.1461
167 307.1535 306.1462
168 307.1535 306.1462
169 307.1536 306.1463
170 307.1537 306.1464
171 307.1538 306.1465
172 307.1889 306.1816
173 307.1893 306.1820
174 307.1895 306.1822
175 307.1897 306.1824
176 307.1902 306.1829
177 307.1903 306.1830
178 307.1904 306.1831
179 307.1904 306.1831
180 307.1904 306.1831
181 307.1905 306.1832
182 307.1905 306.1832
183 307.1906 306.1833
184 309.0054 307.9981
185 309.0663 308.0590
186 309.0948 308.0875
187 309.0949 308.0876
188 309.0951 308.0878
189 309.0951 308.0878
190 309.0951 308.0878
191 309.0951 308.0878
192 309.0953 308.0880
193 309.0954 308.0881
194 309.0962 308.0889
195 309.1316 308.1243
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