MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4187 752.3529 2254.0369
4188 568.2722 2269.0597
4193 764.4179 2290.2319
4196 768.0650 2301.1732
4197 578.7904 2311.1325
4199 777.7125 2330.1157
4200 780.0832 2337.2278
4201 782.0940 2343.2602
4202 786.0724 2355.1954
4203 786.4064 2356.1974
4206 597.5730 2386.2629
4207 796.4291 2386.2655
4208 597.5739 2386.2665
4209 1194.1409 2386.2672
4210 796.4299 2386.2679
4211 796.4313 2386.2721
4212 597.5754 2386.2725
4213 597.5759 2386.2745
4214 597.5760 2386.2749
4215 597.5764 2386.2765
4216 600.3188 2397.2461
4218 482.8547 2409.2371
4219 603.3182 2409.2437
4220 604.5723 2414.2601
4221 805.7612 2414.2618
4222 805.7616 2414.2630
4224 604.5746 2414.2693
4228 807.1138 2418.3196
4229 485.6512 2423.2196
4230 809.0775 2424.2107
4231 405.0429 2424.2137
4232 607.0610 2424.2149
4233 485.8503 2424.2151
4235 486.8527 2429.2271
4236 811.0842 2430.2308
4237 487.0540 2430.2336
4238 608.5659 2430.2345
4240 813.0879 2436.2419
4241 813.1318 2436.3736
4243 610.3150 2437.2309
4246 815.0947 2442.2623
4248 611.5735 2442.2649
4249 815.4276 2443.2610
4250 611.8314 2443.2965
4251 491.2480 2451.2036
4252 613.8114 2451.2165
4253 818.4092 2452.2058
4254 614.0591 2452.2073
4255 491.4489 2452.2081
4256 614.0602 2452.2117
4258 615.3125 2457.2209
4259 492.4518 2457.2226
4260 820.7772 2459.3098
4261 616.2968 2461.1581
4262 493.2410 2461.1686
4263 494.6426 2468.1766
4264 618.5729 2470.2625
4267 497.0491 2480.2091
4268 621.0605 2480.2129
4269 829.4346 2485.2820
4270 498.6438 2488.1826
4271 830.7521 2489.2345
4273 832.0827 2493.2263
4274 832.0850 2493.2332
4275 834.4194 2500.2364
4276 837.0856 2508.2350
4278 839.1003 2514.2791
4279 629.5890 2514.3269
4280 839.4066 2515.1980
4281 629.8345 2515.3089
4283 630.0574 2516.2005
4284 505.0636 2520.2816
4285 505.0645 2520.2861
4286 631.0792 2520.2877
4287 505.0661 2520.2941
4288 631.3272 2521.2797
4289 841.7771 2522.3095
4290 633.8009 2531.1745
4291 633.8011 2531.1753
4294 637.0850 2544.3109
4295 637.5532 2546.1837
4296 510.4448 2547.1876
4297 638.3291 2549.2873
4299 638.8250 2551.2709
4300 511.4618 2552.2726
4301 511.4622 2552.2746
4302 852.4948 2554.4626
4303 640.5796 2558.2893
4304 854.1027 2559.2863
4305 854.1037 2559.2893
4306 854.1047 2559.2923
4307 854.1047 2559.2923
4308 854.1057 2559.2953
4309 854.1067 2559.2983
4310 854.1068 2559.2986
4311 854.1070 2559.2992
4312 854.1083 2559.3031
4314 854.7832 2561.3278
4315 855.4357 2563.2853
4316 856.4322 2566.2748
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