MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4024 989.0199 1976.0252
4025 495.0137 1976.0257
4026 989.0202 1976.0258
4027 659.6829 1976.0269
4028 659.6830 1976.0272
4030 659.6846 1976.0320
4031 659.6848 1976.0326
4033 659.6849 1976.0329
4034 659.6852 1976.0338
4037 659.9427 1976.8063
4038 659.9580 1976.8522
4039 495.2244 1976.8685
4040 396.5786 1977.8566
4041 495.4720 1977.8589
4043 496.5014 1981.9765
4045 663.3381 1986.9925
4046 663.3418 1987.0036
4049 663.3450 1987.0132
4052 499.9901 1995.9313
4053 499.9965 1995.9569
4054 400.3992 1996.9596
4055 666.6607 1996.9603
4056 400.6058 1997.9926
4057 667.0089 1998.0049
4059 667.0115 1998.0127
4061 667.3386 1998.9940
4062 501.5000 2001.9709
4063 668.6808 2003.0206
4064 669.0135 2004.0187
4065 669.0141 2004.0205
4066 1003.0176 2004.0206
4067 1003.0197 2004.0248
4068 669.0165 2004.0277
4069 669.0165 2004.0277
4070 669.3583 2005.0531
4072 670.3503 2008.0291
4075 503.2491 2008.9673
4079 670.6857 2009.0353
4082 504.2481 2012.9633
4083 671.9958 2012.9656
4084 1007.4919 2012.9692
4085 403.6015 2012.9711
4086 403.8019 2013.9731
4087 672.3319 2013.9739
4088 504.5008 2013.9741
4091 672.3562 2014.0468
4092 672.6874 2015.0404
4096 674.0037 2018.9893
4097 1011.0000 2019.9854
4098 674.3362 2019.9868
4099 506.0050 2019.9909
4100 675.3570 2023.0492
4101 507.2470 2024.9589
4102 676.3383 2025.9931
4103 676.3423 2026.0051
4104 677.3188 2028.9346
4105 508.2418 2028.9381
4111 678.3370 2031.9892
4112 678.3381 2031.9925
4113 678.3578 2032.0516
4114 509.2568 2032.9981
4117 679.6546 2035.9420
4123 511.2499 2040.9705
4124 511.2517 2040.9777
4126 681.6631 2041.9675
4127 681.6641 2041.9705
4132 1024.4967 2046.9788
4133 683.3336 2046.9790
4134 410.4031 2046.9791
4137 1026.5099 2051.0052
4138 684.9803 2051.9191
4139 685.3600 2053.0582
4140 514.5049 2053.9905
4141 1028.0349 2054.0552
4142 685.6927 2054.0563
4143 515.2409 2056.9345
4144 1030.0192 2058.0238
4150 688.3229 2061.9469
4153 1035.4849 2068.9552
4155 691.9988 2072.9746
4156 1037.9941 2073.9736
4159 693.0234 2076.0484
4160 520.4937 2077.9457
4161 694.6891 2081.0455
4162 695.6474 2083.9204
4164 697.0256 2088.0550
4165 523.4918 2089.9381
4166 697.9881 2090.9425
4167 419.2080 2091.0036
4168 523.7587 2091.0057
4169 698.3430 2092.0072
4172 1062.5267 2123.0388
4176 724.7225 2171.1457
4177 1086.5822 2171.1498
4178 546.0205 2180.0529
4180 737.3715 2209.0927
4182 747.7192 2240.1358
4184 1123.0994 2244.1842
4185 563.2703 2249.0521
4186 752.0754 2253.2044
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