MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3898 620.6451 1858.9135
3899 465.7377 1858.9217
3900 620.9728 1859.8966
3901 620.9800 1859.9182
3902 931.4952 1860.9758
3903 621.3373 1860.9901
3904 621.6655 1861.9747
3905 622.0068 1862.9986
3906 622.0074 1863.0004
3907 622.3385 1863.9937
3908 934.9760 1867.9374
3909 623.9883 1868.9431
3910 468.7359 1870.9145
3911 468.7376 1870.9213
3912 624.9810 1871.9212
3913 624.9819 1871.9239
3914 468.9886 1871.9253
3915 624.9906 1871.9500
3916 937.7437 1873.4728
3917 938.0750 1874.1354
3918 625.9637 1874.8693
3919 938.4439 1874.8732
3920 625.9651 1874.8735
3921 626.0085 1875.0037
3922 938.9408 1875.8670
3923 626.6339 1876.8799
3924 470.2274 1876.8805
3926 940.4635 1878.9124
3928 629.3224 1884.9454
3929 629.6306 1885.8700
3930 945.9429 1889.8712
3932 316.0081 1890.0049
3933 946.4435 1890.8724
3934 631.2991 1890.8755
3935 474.9662 1895.8357
3936 949.4371 1896.8596
3937 633.6212 1897.8418
3939 952.4387 1902.8628
3940 952.4399 1902.8652
3941 952.4493 1902.8840
3942 953.9387 1905.8628
3944 636.2961 1905.8665
3948 637.9606 1910.8600
3949 956.9174 1911.8202
3950 478.9636 1911.8253
3952 638.6151 1912.8235
3953 638.6153 1912.8241
3954 638.9532 1913.8378
3955 640.2856 1917.8350
3956 959.9404 1917.8662
3957 960.9443 1919.8740
3958 640.9737 1919.8993
3960 481.2327 1920.9017
3961 481.2331 1920.9033
3962 641.6261 1921.8565
3965 482.9619 1927.8185
3966 643.6136 1927.8190
3967 643.9529 1928.8369
3970 967.9390 1933.8634
3971 484.9535 1935.7849
3972 646.2697 1935.7873
3974 969.9337 1937.8528
3975 970.4354 1938.8562
3976 970.4580 1938.9014
3977 485.7327 1938.9017
3978 647.3081 1938.9025
3979 647.3099 1938.9079
3981 485.9608 1939.8141
3983 485.9841 1939.9073
3984 647.9465 1940.8177
3985 649.2795 1944.8167
3986 487.2117 1944.8177
3988 390.5948 1947.9376
3989 650.3217 1947.9433
3991 390.5964 1947.9456
3992 487.9942 1947.9477
3993 488.2095 1948.8089
3994 488.2432 1948.9437
3996 488.4491 1949.7673
3997 975.9336 1949.8526
3998 975.9351 1949.8556
3999 651.2640 1950.7702
4000 976.9403 1951.8660
4001 652.9345 1955.7817
4003 653.6796 1958.0170
4005 490.7202 1958.8517
4006 980.5121 1959.0096
4007 654.0118 1959.0136
4008 654.0125 1959.0157
4009 490.9588 1959.8061
4010 654.6088 1960.8046
4011 655.2950 1962.8632
4014 658.0114 1971.0124
4015 659.3471 1975.0195
4017 659.3510 1975.0312
4018 659.3517 1975.0333
4019 494.9581 1975.8033
4020 659.6804 1976.0194
4022 659.6818 1976.0236
4023 659.6822 1976.0248
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