MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2927 332.5131 994.5175 994.5270 -9.54 1 2 1.6 +1Score > 17 indicates identity RFTMELAK
3329 331.6708 1322.6541 1322.6401 10.6 1 2 2.2 +1Score > 18 indicates identity SFTMSNPNRIR + Deamidated (NQ)
4108 407.2065 2030.9961 2030.9909 2.57 1 2 2.8 +1Score > 19 indicates identity AVTDSDEPPVVRYDVQNK
3711 576.2789 1725.8149 1725.7846 17.5 0 2 1.5 +1Score > 17 indicates identity DEVFFNQTVENVQR + 2 Deamidated (NQ)
4058 1000.0103 1998.0060 1997.9707 17.7 0 2 2.7 +1Score > 19 indicates identity GQNNLWNNGGIQYAPPVR + Deamidated (NQ)
3364 337.4406 1345.7333 1345.7282 3.81 0 2 1.6 +1Score > 17 indicates identity GEYLPIVELWK
4080 670.6863 2009.0371 2009.0542 -8.51 0 2 2.4 +1Score > 19 indicates identity LNGGQVSLPGTLDATSINPR
4119 680.0159 2037.0259 2037.0275 -0.78 0 2 2.6 +1Score > 19 indicates identity MQTVMQTLLPYLNQALR + 2 Deamidated (NQ); Oxidation (M)
2903 493.7738 985.5330 985.5192 14.0 0 2 1.7 +1Score > 17 indicates identity VAVGLEQNR + Deamidated (NQ)
3586 728.3693 1454.7240 1454.7405 -11.3 0 2 2.4 +1Score > 19 indicates identity SFEPLENALAHVK + Deamidated (NQ)
3481 349.4426 1393.7413 1393.7202 15.2 0 2 1.5 +1Score > 17 indicates identity IDVPQQTHDTLK
2519 322.1652 642.3158 642.3085 11.4 0 2 0.59 +1Score > 13 indicates identity HTTER
4452 1047.8401 3140.4985 3140.4617 11.7 1 2 2.8 +1Score > 19 indicates identity VTSNSVMNSFFIPRLGSQIYAMAGMQTR + 3 Deamidated (NQ); 2 Oxidation (M)
2727 432.7039 863.3932 863.4025 -10.7 0 2 1.2 +1Score > 16 indicates identity VDYPGGEK
3124 592.3156 1182.6166 1182.6033 11.3 0 2 1.6 +1Score > 17 indicates identity GITSGFAFLDR
3784 442.7285 1766.8849 1766.8621 12.9 1 2 0.96 +1Score > 20 indicates identity
Score > 15 indicates homology		 VFIGSCTNSRIEDLR + Deamidated (NQ)
3331 331.6710 1322.6549 1322.6401 11.2 1 2 2.4 +1Score > 18 indicates identity SFTMSNPNRIR + Deamidated (NQ)
4293 846.4190 2536.2352 2536.2809 -18.0 1 2 2.8 +1Score > 19 indicates identity GTIAEDLNDGLTGGFKGDNFLLIR + Deamidated (NQ)
3708 859.8958 1717.7770 1717.7876 -6.12 1 2 1.3 +1Score > 16 indicates identity SMQLLAEDRTDHMR + Oxidation (M)
4048 994.5131 1987.0116 1987.0053 3.19 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology		 ACTQPVLGICLGMQLLGR + Deamidated (NQ)
3088 558.7726 1115.5306 1115.5281 2.29 0 2 1.9 +1Score > 17 indicates identity VADDAPLMER
2869 323.4953 967.4641 967.4471 17.5 0 2 2 +1Score > 18 indicates identity ANIGHGDQR + Deamidated (NQ)
3995 488.2444 1948.9485 1948.9247 12.2 1 2 0.99 +1Score > 20 indicates identity
Score > 15 indicates homology		 SDNMIADTVFRMIGHAR + Oxidation (M)
4089 672.3393 2013.9961 2014.0346 -19.1 0 2 2.5 +1Score > 19 indicates identity IATPLGPLWVICDEQFR
3213 629.2761 1256.5376 1256.5244 10.5 0 2 0.81 +1Score > 14 indicates identity SWDACVNSYR
3212 628.3652 1254.7158 1254.7044 9.13 1 2 1.2 +1Score > 15 indicates identity ERQAIEQLIR
2977 515.3040 1028.5934 1028.5978 -4.27 0 2 1.8 +1Score > 17 indicates identity LVLTTQAQR
3946 954.4411 1906.8676 1906.8771 -4.96 0 2 2.2 +1Score > 18 indicates identity IAFLFTGQGSQYVNMGR + 3 Deamidated (NQ); Oxidation (M)
4021 659.6807 1976.0203 1976.0288 -4.32 1 2 3 +1Score > 19 indicates identity MNLAIQILASYPPSGKEK + Deamidated (NQ); Oxidation (M)
4115 678.6910 2033.0512 2033.0326 9.15 0 2 2.2 +1Score > 18 indicates identity VLMQDFTGVPAVVDLAAMR + Deamidated (NQ)
2936 502.2544 1002.4942 1002.5094 -15.1 1 2 3.1 +1Score > 19 indicates identity DRDQTLVR + Deamidated (NQ)
4078 670.6853 2009.0341 2009.0694 -17.6 1 2 2.8 +1Score > 19 indicates identity RDNLEISPNLWAGVGLVR + Deamidated (NQ)
2857 478.2633 954.5120 954.5107 1.37 1 2 1.7 +1Score > 17 indicates identity ADGGRRPAR
3795 592.6426 1774.9060 1774.8751 17.4 1 2 2.8 +1Score > 19 indicates identity RQLPWTNAFADAQTR + Deamidated (NQ)
3892 617.6649 1849.9729 1849.9549 9.72 0 2 2.2 +1Score > 18 indicates identity AFTNYLPGFNIPMLPR
3969 967.9380 1933.8614 1933.8298 16.3 1 2 1.6 +1Score > 16 indicates identity QSMGFDTNMEGGFLARR + 2 Deamidated (NQ); Oxidation (M)
2636 360.7067 719.3988 719.3966 3.09 0 2 1.9 +1Score > 17 indicates identity GEVLFR
2897 985.4759 984.4686 984.4665 2.18 0 2 1.3 +1Score > 15 indicates identity GTYPAYSAR
4170 701.3167 2100.9283 2100.8887 18.8 1 2 1.4 +1Score > 16 indicates identity EWYFANNYIYDMGRNK + 2 Deamidated (NQ); Oxidation (M)
3439 345.4383 1377.7241 1377.7252 -0.79 0 2 2.3 +1Score > 18 indicates identity KPDHQLAALQEK + Deamidated (NQ)
3541 476.5827 1426.7263 1426.7092 12.0 0 2 3.1 +1Score > 19 indicates identity LGIPDEALHNYGK + Deamidated (NQ)
4095 673.0226 2016.0460 2016.0105 17.6 1 2 2.5 +1Score > 18 indicates identity SPLYQKQFYQPFINSR + Deamidated (NQ)
2957 507.7613 1013.5080 1013.5142 -6.03 0 2 1.7 +1Score > 17 indicates identity LPGELSGGQR + Deamidated (NQ)
4110 1016.5120 2031.0094 2031.0459 -17.9 0 2 3.1 +1Score > 19 indicates identity NVGDRPLLWSTLGQSLMK + Deamidated (NQ); Oxidation (M)
2892 492.7156 983.4166 983.4165 0.19 0 2 0.67 +1Score > 13 indicates identity TTCSLCSR
4455 1062.1829 3183.5269 3183.5191 2.44 1 2 3 +1Score > 19 indicates identity IAATMENAQKGEIMPNIPQMSAFWYAVR + Deamidated (NQ); Oxidation (M)
2876 486.8046 971.5946 971.6127 -18.6 1 2 0.68 +1Score > 13 indicates identity TSIARLLAK
2707 414.2061 826.3976 826.4086 -13.2 1 2 1.6 +1Score > 16 indicates identity REFYGR
3679 559.6218 1675.8436 1675.8716 -16.7 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 GFPGVLAAADAMVKTGR + Oxidation (M)
3271 643.3593 1284.7040 1284.7224 -14.3 1 2 3 +1Score > 19 indicates identity MIIGPVKNIER + Oxidation (M)
3524 475.5824 1423.7254 1423.7235 1.32 0 2 2.6 +1Score > 18 indicates identity VFVEVFDEEALK
3348 669.8168 1337.6190 1337.6260 -5.23 0 2 2.1 +1Score > 17 indicates identity WCAFLLQMPR + Deamidated (NQ); Oxidation (M)
4412 924.1354 2769.3844 2769.3358 17.6 1 2 3.2 +1Score > 19 indicates identity NVVVLAGNHEINFNGNYTARLANHK + 5 Deamidated (NQ)
3042 537.2560 1072.4974 1072.4938 3.44 1 2 1.1 +1Score > 14 indicates identity KDFNSYGSR
2731 435.7583 869.5020 869.5083 -7.16 1 2 1 +1Score > 14 indicates identity ERLLSPR
3086 370.1584 1107.4534 1107.4655 -11.0 0 2 0.73 +1Score > 13 indicates identity AMLDGQDWR + Deamidated (NQ); Oxidation (M)
3363 337.4405 1345.7329 1345.7136 14.3 1 2 1.9 +1Score > 17 indicates identity MSELIVSRQQR
3947 956.4371 1910.8596 1910.8792 -10.2 0 2 2.7 +1Score > 18 indicates identity ALNSVEASQPHQDQMEK
4093 672.6927 2015.0563 2015.0397 8.21 1 2 2.7 +1Score > 18 indicates identity NTLWNQIKANMLDIPVK + 2 Deamidated (NQ); Oxidation (M)
2585 688.3452 687.3379 687.3300 11.5 0 2 1 +1Score > 14 indicates identity QSGPGSR
2919 496.7774 991.5402 991.5372 3.09 1 2 1.9 +1Score > 17 indicates identity LNKMGIATK + Deamidated (NQ); Oxidation (M)
3762 438.9881 1751.9233 1751.9431 -11.3 1 2 2.3 +1Score > 18 indicates identity GFHVERDQVGNILIR
4292 844.7336 2531.1790 2531.2292 -19.9 1 2 2.7 +1Score > 18 indicates identity QYGDFENGIPVHDTIARVVSQGK + 2 Deamidated (NQ)
4035 659.6852 1976.0338 1976.0075 13.3 1 1 3.2 +1Score > 19 indicates identity EPRANTQAAEHILQEIR + Deamidated (NQ)
2800 929.5177 928.5104 928.5090 1.53 1 1 2.7 +1Score > 18 indicates identity RLEQIDR
3405 341.9435 1363.7449 1363.7473 -1.76 1 1 0.94 +1Score > 14 indicates identity FRHGIAIAGTHGK
3742 581.6658 1741.9756 1741.9661 5.41 1 1 1.1 +1Score > 14 indicates identity MIAHLQKLSFGQVVR + Oxidation (M)
4410 921.4666 2761.3780 2761.4147 -13.3 1 1 3 +1Score > 19 indicates identity DPLHQFASQQTPEAQLQALQDKIR
2735 437.2448 872.4750 872.4603 16.9 0 1 3.6 +1Score > 19 indicates identity INAAQDLK + Deamidated (NQ)
3426 456.2560 1365.7462 1365.7728 -19.5 1 1 1.3 +1Score > 15 indicates identity QRDGAPAGILQIK
2847 318.5056 952.4950 952.4800 15.7 1 1 2.6 +1Score > 18 indicates identity FGGMKIER + Oxidation (M)
3158 406.8932 1217.6578 1217.6438 11.5 0 1 2.3 +1Score > 18 indicates identity MVVGEGLLSAAR + Oxidation (M)
3618 744.3301 1486.6456 1486.6697 -16.2 1 1 1.4 +1Score > 15 indicates identity MDSFRDVWMLR + 2 Oxidation (M)
4050 995.0073 1988.0000 1988.0135 -6.79 0 1 3.5 +1Score > 19 indicates identity TLTIAAPGAASMQLAENALK + 2 Deamidated (NQ); Oxidation (M)
2543 332.7039 663.3932 663.3955 -3.45 0 1 0.97 +1Score > 14 indicates identity GLAYIK
3382 340.9359 1359.7145 1359.6929 15.9 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 AMTPVRALSGGER + Oxidation (M)
4074 503.0151 2008.0313 2008.0009 15.1 1 1 3.1 +1Score > 19 indicates identity FCQELGKQIPVAMTLEK + Deamidated (NQ); Oxidation (M)
3065 363.4875 1087.4407 1087.4352 4.99 0 1 0.76 +1Score > 13 indicates identity CHQNATAER + 2 Deamidated (NQ)
4122 680.0188 2037.0346 2037.0643 -14.6 1 1 3.3 +1Score > 19 indicates identity LTPEQWAQAGRLIAAGTPR + 2 Deamidated (NQ)
3236 423.5234 1267.5484 1267.5689 -16.2 0 1 1.5 +1Score > 15 indicates identity NVMDMFIDGAR
4277 837.0870 2508.2392 2508.2240 6.06 1 1 0.84 +1Score > 20 indicates identity
Score > 13 indicates homology		 MDKIFVDEAVNELQTIQDMLR + Deamidated (NQ)
4226 604.5762 2414.2757 2414.2378 15.7 1 1 2.4 +1Score > 18 indicates identity FVEGKPLMLEDSYMPVKLFR + Oxidation (M)
3543 714.3710 1426.7274 1426.7092 12.8 0 1 3.4 +1Score > 19 indicates identity LGIPDEALHNYGK + Deamidated (NQ)
3553 476.5844 1426.7314 1426.7052 18.4 0 1 3.6 +1Score > 19 indicates identity LQQIEPNAGTEAR + Deamidated (NQ)
3703 572.3332 1713.9778 1713.9600 10.4 1 1 0.88 +1Score > 13 indicates identity ILLIMGEWLAVQRR + Deamidated (NQ); Oxidation (M)
2920 496.7776 991.5406 991.5372 3.49 1 1 2.2 +1Score > 17 indicates identity LNKMGIATK + Deamidated (NQ); Oxidation (M)
4205 593.0727 2368.2617 2368.2386 9.75 1 1 2.2 +1Score > 17 indicates identity GNAAELFSGIRHIAINILTNDK + 2 Deamidated (NQ)
3752 584.6448 1750.9126 1750.9002 7.04 0 1 2.6 +1Score > 18 indicates identity VNNFQVSAASTPFLTR
3178 310.6449 1238.5505 1238.5278 18.4 0 1 1.7 +1Score > 16 indicates identity SYMYDFIQR + Deamidated (NQ); Oxidation (M)
3381 340.9357 1359.7137 1359.6929 15.3 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 AMTPVRALSGGER + Oxidation (M)
3645 768.9084 1535.8022 1535.7952 4.58 1 1 2.4 +1Score > 17 indicates identity LIMGFAPAMLERR + 2 Oxidation (M)
3408 341.9442 1363.7477 1363.7473 0.29 1 1 1.1 +1Score > 14 indicates identity FRHGIAIAGTHGK
2997 520.7262 1039.4378 1039.4427 -4.63 1 1 0.78 +1Score > 13 indicates identity VNDTMCKR + Deamidated (NQ); Oxidation (M)
3445 345.9319 1379.6985 1379.6721 19.1 0 1 2.4 +1Score > 17 indicates identity FPYVNANVIDAR + 2 Deamidated (NQ)
3505 470.2529 1407.7369 1407.7510 -10.1 0 1 2.4 +1Score > 17 indicates identity HAAQGIIDWLGVK + Deamidated (NQ)
4163 522.2385 2084.9249 2084.9592 -16.5 0 1 2 +1Score > 16 indicates identity DGFLQPSIQAFNQYWDR + Deamidated (NQ)
4392 896.7653 2687.2741 2687.2717 0.90 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 RLMTEQHQGLGAAIISNMEQVCAK + 3 Deamidated (NQ)
4453 1061.5312 3181.5718 3181.5472 7.72 1 1 3.1 +1Score > 18 indicates identity RAVFLGMLLQAMQQFTGMNIIMYYAPR + 3 Deamidated (NQ); Oxidation (M)
2969 510.7743 1019.5340 1019.5321 1.89 0 1 3.2 +1Score > 19 indicates identity DMVLATLNK + Oxidation (M)
3760 438.9878 1751.9221 1751.8941 16.0 0 1 2.7 +1Score > 18 indicates identity LAEPAPTGEQLQNILR + 3 Deamidated (NQ)
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