MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3785 589.9692 1766.8858
3786 591.0044 1769.9914
3787 591.0050 1769.9932
3788 591.0054 1769.9944
3789 591.2405 1770.6997
3790 591.3371 1770.9895
3792 592.3016 1773.8830
3793 887.9558 1773.8970
3794 444.7273 1774.8801
3798 593.9794 1778.9164
3799 445.7368 1778.9181
3800 593.9802 1778.9188
3802 595.2523 1782.7351
3803 596.6618 1786.9636
3804 448.2224 1788.8605
3805 597.2942 1788.8608
3806 597.3327 1788.9763
3807 597.3342 1788.9808
3808 597.6284 1789.8634
3809 895.9986 1789.9826
3810 896.0000 1789.9854
3812 597.6700 1789.9882
3813 598.2362 1791.6868
3814 598.5652 1792.6738
3815 449.1758 1792.6741
3816 449.1802 1792.6917
3817 598.5959 1792.7659
3818 598.6150 1792.8232
3819 449.4633 1793.8241
3820 599.3012 1794.8818
3821 600.6307 1798.8703
3823 903.4950 1804.9754
3824 602.6667 1804.9783
3825 602.6673 1804.9801
3826 602.6675 1804.9807
3827 602.9979 1805.9719
3828 903.9937 1805.9728
3829 603.0001 1805.9785
3830 903.9973 1805.9800
3831 603.0013 1805.9821
3832 603.0015 1805.9827
3833 603.0026 1805.9860
3834 604.9814 1811.9224
3835 453.9960 1811.9549
3836 604.9961 1811.9665
3837 605.2922 1812.8548
3838 454.4712 1813.8557
3839 454.7287 1814.8857
3840 606.6669 1816.9789
3841 606.6683 1816.9831
3842 909.4993 1816.9840
3844 607.0021 1817.9845
3846 607.9979 1820.9719
3847 607.9985 1820.9737
3848 911.4948 1820.9750
3849 608.3300 1821.9682
3850 913.4604 1824.9062
3852 457.7401 1826.9313
3853 457.7409 1826.9345
3854 609.9905 1826.9497
3855 610.3196 1827.9370
3856 457.9932 1827.9437
3857 457.9940 1827.9469
3858 610.3233 1827.9481
3860 610.3267 1827.9583
3861 610.6497 1828.9273
3862 610.9858 1829.9356
3863 611.9966 1832.9680
3864 611.9977 1832.9713
3865 917.4963 1832.9780
3866 917.9910 1833.9674
3867 612.3321 1833.9745
3868 917.9950 1833.9754
3869 612.3331 1833.9775
3870 612.3331 1833.9775
3871 613.9875 1838.9407
3872 614.3190 1839.9352
3873 920.9756 1839.9366
3874 615.3130 1842.9172
3875 461.7366 1842.9173
3876 461.7383 1842.9241
3877 615.3156 1842.9250
3878 461.7389 1842.9265
3879 922.4773 1842.9400
3880 922.9672 1843.9198
3881 461.9901 1843.9313
3882 923.4874 1844.9602
3883 923.5214 1845.0282
3885 616.3426 1846.0060
3886 616.6762 1847.0068
3887 616.6772 1847.0098
3888 925.4750 1848.9354
3889 925.4956 1848.9766
3890 463.4911 1849.9353
3891 617.6529 1849.9369
3893 619.3254 1854.9544
3894 464.9926 1855.9413
3895 619.9606 1856.8600
3896 465.7294 1858.8885
3897 465.7354 1858.9125
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