MASCOT Search Results

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User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3648 774.8401 1547.6656
3649 774.8425 1547.6704
3651 518.8802 1553.6188
3652 522.2886 1563.8440
3653 782.9299 1563.8452
3654 522.2899 1563.8479
3655 523.5424 1567.6054
3656 392.9091 1567.6073
3657 393.1580 1568.6029
3658 797.4500 1592.8854
3659 801.8824 1601.7502
3661 814.4246 1626.8346
3662 545.2958 1632.8656
3664 818.4117 1634.8088
3665 819.4561 1636.8976
3666 819.9521 1637.8896
3668 819.9588 1637.9030
3670 549.6221 1645.8445
3671 550.6285 1648.8637
3672 830.4241 1658.8336
3675 835.4083 1668.8020
3676 557.6524 1669.9354
3677 837.4468 1672.8790
3678 559.3079 1674.9019
3680 419.9694 1675.8485
3681 559.9602 1676.8588
3682 559.9604 1676.8594
3685 566.3260 1695.9562
3686 850.3887 1698.7628
3687 850.3979 1698.7812
3688 857.4866 1712.9586
3689 571.9950 1712.9632
3690 429.2485 1712.9649
3691 857.4899 1712.9652
3692 571.9962 1712.9668
3693 857.4929 1712.9712
3694 571.9977 1712.9713
3695 857.4930 1712.9714
3696 857.4935 1712.9724
3697 857.4944 1712.9742
3698 571.9988 1712.9746
3702 572.3329 1713.9769
3705 858.9036 1715.7926
3706 859.3964 1716.7782
3707 573.2728 1716.7966
3709 863.4197 1724.8248
3710 863.9132 1725.8118
3712 863.9172 1725.8198
3714 576.6140 1726.8202
3715 576.6339 1726.8799
3717 577.3286 1728.9640
3718 865.9409 1729.8672
3719 866.8800 1731.7454
3720 867.3998 1732.7850
3728 869.8913 1737.7680
3729 869.9395 1737.8644
3730 870.3870 1738.7594
3731 580.5962 1738.7668
3732 580.9244 1739.7514
3733 871.4911 1740.9676
3734 581.3309 1740.9709
3735 581.3315 1740.9727
3736 581.3324 1740.9754
3737 871.9119 1741.8092
3738 581.6638 1741.9696
3739 871.9932 1741.9718
3740 871.9932 1741.9718
3741 871.9938 1741.9730
3744 581.6672 1741.9798
3745 872.9034 1743.7922
3746 873.3922 1744.7698
3747 874.4940 1746.9734
3748 874.8792 1747.7438
3749 874.9023 1747.7900
3753 584.6468 1750.9186
3754 438.7373 1750.9201
3755 876.9637 1751.9128
3757 351.3907 1751.9171
3758 876.9669 1751.9192
3759 584.9813 1751.9221
3761 584.9817 1751.9233
3763 877.4199 1752.8252
3764 877.4909 1752.9672
3765 877.8702 1753.7258
3766 585.5873 1753.7401
3767 877.9153 1753.8160
3768 878.3696 1754.7246
3769 585.9163 1754.7271
3771 878.9452 1755.8758
3773 879.9733 1757.9320
3774 586.9855 1757.9347
3775 880.8846 1759.7546
3776 588.6005 1762.7797
3777 588.9290 1763.7652
3778 441.9525 1763.7809
3779 588.9966 1763.9680
3780 883.3883 1764.7620
3781 589.2629 1764.7669
3782 442.1990 1764.7669
3783 589.3326 1764.9760
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