MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3193 622.2826 1242.5506
3195 415.1917 1242.5533
3196 623.7827 1245.5508
3197 416.1911 1245.5515
3199 625.8421 1249.6696
3202 418.5361 1252.5865
3204 627.3649 1252.7152
3206 418.5798 1252.7176
3208 628.2562 1254.4978
3209 419.1739 1254.4999
3214 315.1420 1256.5389
3215 419.8536 1256.5390
3216 419.8536 1256.5390
3217 315.1421 1256.5393
3218 629.2773 1256.5400
3219 419.8795 1256.6167
3223 420.5442 1258.6108
3225 631.7647 1261.5148
3226 422.1740 1263.5002
3227 422.5057 1264.4953
3229 423.2118 1266.6136
3230 634.3539 1266.6932
3231 423.2391 1266.6955
3233 634.3659 1266.7172
3234 634.7719 1267.5292
3238 424.8413 1271.5021
3239 637.2595 1272.5044
3240 425.9029 1274.6869
3241 638.3560 1274.6974
3242 638.8213 1275.6280
3243 426.5665 1276.6777
3244 320.3778 1277.4821
3245 426.8347 1277.4823
3247 640.3111 1278.6076
3249 640.3125 1278.6104
3251 640.3130 1278.6114
3252 640.3130 1278.6114
3253 640.3141 1278.6136
3254 640.3141 1278.6136
3255 640.3144 1278.6142
3256 640.3146 1278.6146
3257 640.3146 1278.6146
3258 640.3166 1278.6186
3261 427.8397 1280.4973
3262 641.3636 1280.7126
3263 641.3669 1280.7192
3264 428.8405 1283.4997
3265 642.7574 1283.5002
3266 429.1855 1284.5347
3267 643.3019 1284.5892
3269 429.2297 1284.6673
3270 643.3419 1284.6692
3273 643.3611 1284.7076
3274 643.3614 1284.7082
3275 643.3617 1284.7088
3277 643.3638 1284.7130
3278 644.3610 1286.7074
3283 647.3203 1292.6260
3284 324.1724 1292.6605
3285 431.8949 1292.6629
3286 648.2486 1294.4826
3287 432.5021 1294.4845
3288 651.2587 1300.5028
3289 434.5083 1300.5031
3290 651.2993 1300.5840
3294 435.1975 1302.5707
3295 435.1982 1302.5728
3296 327.4192 1305.6477
3297 653.8400 1305.6654
3299 654.3109 1306.6072
3303 654.3496 1306.6846
3308 655.8688 1309.7230
3310 656.3679 1310.7212
3311 657.3499 1312.6852
3312 657.3505 1312.6864
3313 657.3578 1312.7010
3315 657.3598 1312.7050
3316 657.8273 1313.6400
3317 657.8274 1313.6402
3318 439.1974 1314.5704
3322 659.2863 1316.5580
3323 659.2875 1316.5604
3324 659.7867 1317.5588
3325 662.2928 1322.5710
3328 662.3343 1322.6540
3330 441.8921 1322.6545
3333 662.3355 1322.6564
3334 441.8976 1322.6710
3335 662.3552 1322.6958
3337 662.7968 1323.5790
3338 443.7427 1328.2063
3342 666.2954 1330.5762
3343 666.3579 1330.7012
3344 667.2675 1332.5204
3345 445.1820 1332.5242
3346 668.8235 1335.6324
3349 447.1818 1338.5236
3350 670.2745 1338.5344
3352 670.7946 1339.5746
3353 670.8408 1339.6670
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