MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3045 358.8236 1073.4490
3046 359.4843 1075.4311
3047 538.7233 1075.4320
3048 539.2850 1076.5554
3049 540.1869 1078.3592
3050 360.7958 1079.3656
3051 360.8268 1079.4586
3052 360.8278 1079.4616
3054 361.8436 1082.5090
3055 542.2831 1082.5516
3056 542.2969 1082.5792
3057 361.8676 1082.5810
3058 1084.5842 1083.5769
3059 542.7965 1083.5784
3060 542.7980 1083.5814
3061 542.7987 1083.5828
3062 542.7992 1083.5838
3063 543.7458 1085.4770
3064 363.1767 1086.5083
3066 546.7525 1091.4904
3067 546.7574 1091.5002
3068 365.4920 1093.4542
3069 547.7344 1093.4542
3070 365.4963 1093.4671
3071 548.2199 1094.4252
3072 365.8159 1094.4259
3073 548.8130 1095.6114
3074 548.8135 1095.6124
3075 548.8152 1095.6158
3076 548.8156 1095.6166
3078 367.8536 1100.5390
3079 551.2777 1100.5408
3080 368.1505 1101.4297
3081 551.7267 1101.4388
3082 368.8136 1103.4190
3084 369.8442 1106.5108
3087 370.4940 1108.4602
3089 372.8515 1115.5327
3090 559.7092 1117.4038
3091 373.4763 1117.4071
3092 373.5096 1117.5070
3093 562.7198 1123.4250
3094 375.4826 1123.4260
3096 378.4643 1132.3711
3097 567.2776 1132.5406
3098 567.3200 1132.6254
3099 567.8028 1133.5910
3100 570.8069 1139.5992
3101 380.8745 1139.6017
3102 381.8826 1142.6260
3103 381.8828 1142.6266
3106 573.2766 1144.5386
3107 573.8260 1145.6374
3108 576.3084 1150.6022
3109 577.3015 1152.5884
3110 577.3263 1152.6380
3119 586.3126 1170.6106
3120 586.3138 1170.6130
3121 588.3195 1174.6244
3123 394.8472 1181.5198
3125 592.3191 1182.6236
3126 592.7955 1183.5764
3127 395.5332 1183.5778
3128 395.8613 1184.5621
3129 395.8628 1184.5666
3130 395.8719 1184.5939
3131 395.8726 1184.5960
3132 595.3220 1188.6294
3133 595.3229 1188.6312
3134 397.2178 1188.6316
3136 397.2185 1188.6337
3137 598.8247 1195.6348
3138 400.5350 1198.5832
3139 600.2990 1198.5834
3140 300.6532 1198.5837
3141 400.5354 1198.5844
3142 400.8665 1199.5777
3150 401.5368 1201.5886
3152 602.3391 1202.6636
3153 602.7955 1203.5764
3155 603.8088 1205.6030
3156 404.4983 1210.4731
3157 405.5227 1213.5463
3159 609.8362 1217.6578
3165 612.8222 1223.6298
3168 409.5255 1225.5547
3169 409.8666 1226.5780
3171 615.8006 1229.5866
3172 615.8019 1229.5892
3174 616.3020 1230.5894
3177 413.1818 1236.5236
3179 413.8575 1238.5507
3181 414.1855 1239.5347
3182 414.1861 1239.5365
3183 620.7794 1239.5442
3184 620.8426 1239.6706
3185 414.5276 1240.5610
3189 621.8315 1241.6484
3190 621.8325 1241.6504
3191 415.1889 1242.5449
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