MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4435)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2891 983.5362 982.5289
2893 328.8141 983.4205
2894 328.8142 983.4208
2895 492.7383 983.4620
2896 493.2276 984.4406
2898 985.5167 984.5094
2899 493.2620 984.5094
2900 985.5176 984.5103
2901 493.2627 984.5108
2904 493.9460 985.8774
2909 988.5107 987.5034
2912 495.5849 989.1552
2913 495.7255 989.4364
2915 992.5419 991.5346
2921 331.8269 992.4589
2922 497.2368 992.4590
2923 497.6095 993.2044
2924 332.1581 993.4525
2925 332.2074 993.6004
2929 333.8320 998.4742
2930 500.2461 998.4776
2931 501.2372 1000.4598
2932 501.2507 1000.4868
2937 502.2664 1002.5182
2940 502.7601 1003.5056
2941 502.7609 1003.5072
2942 335.8093 1004.4061
2943 504.2807 1006.5468
2944 337.0004 1007.9794
2945 337.4978 1009.4716
2946 1010.4871 1009.4798
2948 338.1529 1011.4369
2949 506.7281 1011.4416
2950 338.1548 1011.4426
2952 338.1612 1011.4618
2953 506.7382 1011.4618
2956 507.2274 1012.4402
2959 339.1776 1014.5110
2961 508.7563 1015.4980
2962 1016.5057 1015.4984
2963 508.7565 1015.4984
2964 509.0053 1015.9960
2965 509.2399 1016.4652
2966 509.7372 1017.4598
2967 1020.5397 1019.5324
2968 510.7737 1019.5328
2970 341.4892 1021.4458
2971 512.7574 1023.5002
2972 342.8240 1025.4502
2974 342.8245 1025.4517
2976 342.8263 1025.4571
2978 344.1686 1029.4840
2979 515.7493 1029.4840
2980 344.1692 1029.4858
2982 344.4840 1030.4302
2983 516.3036 1030.5926
2984 516.7411 1031.4676
2986 345.4680 1033.3822
2987 518.7558 1035.4970
2988 518.7560 1035.4974
2989 518.7579 1035.5012
2990 518.7581 1035.5016
2991 346.1747 1035.5023
2993 346.8302 1037.4688
2995 520.2156 1038.4166
2996 520.7261 1039.4376
2998 347.8118 1040.4136
2999 521.2146 1040.4146
3001 521.7626 1041.5106
3002 521.7632 1041.5118
3003 1042.5194 1041.5121
3004 521.7640 1041.5134
3005 521.7648 1041.5150
3006 349.1563 1044.4471
3007 523.2332 1044.4518
3011 524.7205 1047.4264
3012 350.1601 1047.4585
3013 524.7729 1047.5312
3014 525.2872 1048.5598
3015 525.2878 1048.5610
3016 350.8093 1049.4061
3017 351.1401 1050.3985
3018 527.7305 1053.4464
3019 527.7307 1053.4468
3020 352.1570 1053.4492
3022 352.4904 1054.4494
3023 353.4998 1057.4776
3024 529.7466 1057.4786
3025 353.5013 1057.4821
3027 532.7045 1063.3944
3028 355.4724 1063.3954
3029 355.5015 1063.4827
3031 532.7567 1063.4988
3035 356.1650 1065.4732
3036 535.7137 1069.4128
3037 357.4787 1069.4143
3038 535.7601 1069.5056
3039 535.7615 1069.5084
3041 537.2556 1072.4966
3044 358.8007 1073.3803
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