MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2730 435.2689 868.5232
2732 435.7751 869.5356
2733 435.7769 869.5392
2734 871.9120 870.9047
2736 437.6796 873.3446
2738 877.0842 876.0769
2742 884.4615 883.4542
2745 886.4069 885.3996
2746 444.7199 887.4252
2753 446.7682 891.5218
2756 449.7260 897.4374
2757 449.7531 897.4916
2758 449.7734 897.5322
2762 301.6807 902.0203
2765 302.4985 904.4737
2766 453.2447 904.4748
2767 454.2320 906.4494
2769 304.8324 911.4754
2770 456.7452 911.4758
2771 304.8354 911.4844
2772 304.8355 911.4847
2773 456.7498 911.4850
2776 457.2256 912.4366
2777 457.2256 912.4366
2778 457.7398 913.4650
2779 457.7401 913.4656
2781 306.4739 916.3999
2782 459.2285 916.4424
2783 459.7578 917.5010
2784 918.5091 917.5018
2785 459.7582 917.5018
2786 459.7583 917.5020
2787 462.1947 922.3748
2788 462.1949 922.3752
2790 309.1494 924.4264
2791 463.2219 924.4292
2793 309.8007 926.3803
2794 464.1975 926.3804
2795 464.7331 927.4516
2796 310.1582 927.4528
2798 464.7349 927.4552
2802 310.5114 928.5124
2805 310.5119 928.5139
2810 465.9868 929.9590
2811 467.2415 932.4684
2812 467.2424 932.4702
2813 467.2440 932.4734
2814 312.1509 933.4309
2815 467.7427 933.4708
2816 312.8038 935.3896
2817 469.2445 936.4744
2818 313.1740 936.5002
2819 313.1742 936.5008
2820 313.8129 938.4169
2821 470.2163 938.4180
2822 471.2642 940.5138
2824 314.8530 941.5372
2825 314.8532 941.5378
2826 471.7762 941.5378
2827 315.1457 942.4153
2828 472.2159 942.4172
2829 472.2286 942.4426
2830 943.4513 942.4440
2831 472.7329 943.4512
2833 315.8363 944.4871
2838 474.2719 946.5292
2839 317.6606 949.9600
2840 476.2376 950.4606
2842 317.8346 950.4820
2845 318.4969 952.4689
2846 318.4971 952.4695
2848 477.2550 952.4954
2849 319.1527 954.4363
2850 955.4988 954.4915
2851 319.1753 954.5041
2852 319.1761 954.5065
2853 319.1770 954.5092
2858 319.4905 955.4497
2860 478.7430 955.4714
2862 481.7329 961.4512
2863 483.2379 964.4612
2864 483.2386 964.4626
2865 484.2365 966.4584
2866 323.1605 966.4597
2867 323.4879 967.4419
2868 484.7297 967.4448
2870 485.2608 968.5070
2871 485.7536 969.4926
2873 486.2594 970.5042
2874 324.5091 970.5055
2875 324.8131 971.4175
2881 327.8044 980.3914
2882 491.2111 980.4076
2884 491.7213 981.4280
2885 492.2584 982.5022
2886 492.2590 982.5034
2887 328.5089 982.5049
2888 983.5336 982.5263
2889 492.2705 982.5264
2890 983.5339 982.5266
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