MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2561 336.6788 671.3430
2562 336.6891 671.3636
2563 336.6892 671.3638
2565 337.1761 672.3376
2566 337.1830 672.3514
2569 675.3459 674.3386
2571 340.6931 679.3716
2572 340.6935 679.3724
2573 683.8774 682.8701
2574 342.6500 683.2854
2575 342.6568 683.2990
2577 342.6859 683.3572
2578 342.7073 683.4000
2579 343.6865 685.3584
2580 343.6867 685.3588
2581 343.7017 685.3888
2582 343.7026 685.3906
2583 344.1660 686.3174
2584 687.3366 686.3293
2586 345.1597 688.3048
2587 345.1598 688.3050
2591 346.6786 691.3426
2592 693.3483 692.3410
2596 348.6795 695.3444
2597 696.4065 695.3992
2598 348.9421 695.8696
2599 349.6445 697.2744
2600 349.6512 697.2878
2602 700.3651 699.3578
2603 350.6866 699.3586
2607 703.3025 702.2952
2610 352.6603 703.3060
2611 704.3643 703.3570
2613 353.6813 705.3480
2614 707.3873 706.3800
2615 707.4246 706.4173
2616 355.1379 708.2612
2617 709.3192 708.3119
2618 355.6521 709.2896
2619 355.6628 709.3110
2624 357.1528 712.2910
2625 357.1534 712.2922
2626 715.3554 714.3481
2627 358.1814 714.3482
2628 358.1815 714.3484
2629 358.1816 714.3486
2630 715.3560 714.3487
2632 359.6367 717.2588
2633 718.3276 717.3203
2635 720.3334 719.3261
2638 720.4072 719.3999
2639 360.7078 719.4010
2640 360.7078 719.4010
2641 720.4085 719.4012
2642 361.6916 721.3686
2643 725.3969 724.3896
2644 363.2025 724.3904
2646 364.1913 726.3680
2648 364.1922 726.3698
2649 364.1922 726.3698
2653 365.6472 729.2798
2656 369.6600 737.3054
2657 370.1920 738.3694
2658 743.3519 742.3446
2659 743.4451 742.4378
2660 372.6887 743.3628
2661 372.6893 743.3640
2663 374.1360 746.2574
2664 374.7046 747.3946
2665 748.4025 747.3952
2666 374.7054 747.3962
2667 375.1848 748.3550
2668 377.1580 752.3014
2669 755.3717 754.3644
2670 378.2067 754.3988
2671 757.3647 756.3574
2676 383.6359 765.2572
2677 772.3679 771.3606
2681 387.7112 773.4078
2682 782.4070 781.3997
2683 391.7073 781.4000
2686 393.1161 784.2176
2690 403.7313 805.4480
2694 406.7552 811.4958
2699 410.1794 818.3442
2701 411.7304 821.4462
2704 412.7374 823.4602
2705 412.7556 823.4966
2706 413.6956 825.3766
2713 418.7064 835.3982
2714 836.4633 835.4560
2715 836.4656 835.4583
2716 836.4695 835.4622
2717 419.7188 837.4230
2718 421.7053 841.3960
2720 842.5132 841.5059
2725 859.1018 858.0945
2726 430.7353 859.4560
2728 432.7587 863.5028
2729 433.7379 865.4612
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