MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2400 529.2760 528.2687
2401 529.2761 528.2688
2402 529.2762 528.2689
2403 529.2763 528.2690
2407 529.3489 528.3416
2408 530.3309 529.3236
2409 531.1165 530.1092
2411 531.2915 530.2842
2413 533.1055 532.0982
2415 536.3109 535.3036
2416 536.3109 535.3036
2417 537.2338 536.2265
2418 537.2354 536.2281
2419 537.2366 536.2293
2420 537.7319 536.7246
2421 538.2310 537.2237
2422 538.2328 537.2255
2423 539.3311 538.3238
2426 542.2585 541.2512
2429 545.2712 544.2639
2432 550.2332 549.2259
2433 550.2682 549.2609
2434 550.2687 549.2614
2435 550.2693 549.2620
2436 551.2492 550.2419
2437 552.2803 551.2730
2438 552.2810 551.2737
2439 555.2648 554.2575
2440 559.2168 558.2095
2441 559.2666 558.2593
2442 561.2773 560.2700
2444 562.2610 561.2537
2445 563.3303 562.3230
2446 566.2632 565.2559
2447 566.2635 565.2562
2448 567.2446 566.2373
2449 567.2447 566.2374
2450 568.2416 567.2343
2451 568.2424 567.2351
2452 569.1529 568.1456
2453 569.3430 568.3357
2454 569.3434 568.3361
2455 572.2512 571.2439
2459 577.2792 576.2719
2461 578.2636 577.2563
2462 581.2601 580.2528
2463 581.2607 580.2534
2464 582.3085 581.3012
2465 584.2215 583.2142
2466 584.2297 583.2224
2467 588.9945 587.9872
2470 594.2329 593.2256
2471 594.3140 593.3067
2472 300.1893 598.3640
2473 301.1213 600.2280
2474 302.0932 602.1718
2475 302.6086 603.2026
2476 605.2729 604.2656
2477 303.5982 605.1818
2478 303.5985 605.1824
2479 304.1483 606.2820
2480 305.6175 609.2204
2481 305.6885 609.3624
2482 305.6889 609.3632
2483 308.1168 614.2190
2484 308.1169 614.2192
2485 311.0823 620.1500
2486 311.1478 620.2810
2489 312.6599 623.3052
2490 312.7144 623.4142
2491 313.0906 624.1666
2492 313.6855 625.3564
2493 626.3640 625.3567
2494 626.3641 625.3568
2495 315.1066 628.1986
2498 315.6835 629.3524
2504 319.6861 637.3576
2505 638.3651 637.3578
2506 319.6864 637.3582
2507 638.3657 637.3584
2508 321.2878 640.5610
2509 321.6651 641.3156
2510 321.6656 641.3166
2526 651.3510 650.3437
2527 653.3463 652.3390
2528 328.6631 655.3116
2529 329.6706 657.3266
2530 329.6707 657.3268
2532 660.3340 659.3267
2541 664.2534 663.2461
2542 332.6749 663.3352
2544 664.4144 663.4071
2550 668.8223 667.8150
2551 335.6712 669.3278
2554 336.1784 670.3422
2555 336.1789 670.3432
2557 672.3481 671.3408
2558 336.6778 671.3410
2559 336.6784 671.3422
2560 336.6787 671.3428
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