MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2296 457.1420 456.1347
2297 457.2076 456.2003
2298 457.2253 456.2180
2299 457.2254 456.2181
2300 457.2260 456.2187
2301 457.2266 456.2193
2302 457.2783 456.2710
2303 458.2731 457.2658
2304 459.1201 458.1128
2305 459.2417 458.2344
2306 460.3148 459.3075
2307 461.2567 460.2494
2308 461.2568 460.2495
2309 461.2575 460.2502
2310 461.2752 460.2679
2311 463.1401 462.1328
2312 463.2336 462.2263
2313 463.2338 462.2265
2314 464.7476 463.7403
2315 465.2122 464.2049
2316 465.2132 464.2059
2317 465.2470 464.2397
2318 465.2496 464.2423
2319 465.2503 464.2430
2320 466.0446 465.0373
2321 467.0927 466.0854
2322 467.1615 466.1542
2323 467.2269 466.2196
2324 467.2269 466.2196
2325 467.2272 466.2199
2326 467.2278 466.2205
2327 468.2111 467.2038
2328 469.2408 468.2335
2329 469.2412 468.2339
2330 469.2419 468.2346
2331 469.2778 468.2705
2332 471.0943 470.0870
2333 471.1343 470.1270
2334 471.1557 470.1484
2335 472.2914 471.2841
2336 472.3257 471.3184
2337 472.3263 471.3190
2338 472.3276 471.3203
2339 472.7283 471.7210
2340 473.1099 472.1026
2341 473.1356 472.1283
2342 473.1505 472.1432
2343 473.2045 472.1972
2344 474.2684 473.2611
2345 474.2694 473.2621
2346 474.2695 473.2622
2347 474.2700 473.2627
2349 475.2355 474.2282
2350 475.2366 474.2293
2352 475.2907 474.2834
2353 475.3285 474.3212
2354 477.2520 476.2447
2355 479.2289 478.2216
2356 479.2637 478.2564
2357 479.2650 478.2577
2358 479.2654 478.2581
2359 479.2669 478.2596
2360 479.2672 478.2599
2361 481.2632 480.2559
2362 484.2078 483.2005
2363 487.1511 486.1438
2364 493.0183 492.0110
2365 493.0204 492.0131
2366 493.0204 492.0131
2367 493.0207 492.0134
2368 493.0209 492.0136
2369 493.0213 492.0140
2370 493.0214 492.0141
2371 493.1171 492.1098
2372 495.2602 494.2529
2373 495.2603 494.2530
2374 495.2606 494.2533
2375 495.2610 494.2537
2376 496.2534 495.2461
2377 497.1723 496.1650
2378 497.5524 496.5451
2379 501.2377 500.2304
2380 502.2272 501.2199
2382 511.3041 510.2968
2383 513.2794 512.2721
2384 513.2799 512.2726
2385 514.2857 513.2784
2386 515.3330 514.3257
2387 519.2156 518.2083
2388 519.2473 518.2400
2389 519.3174 518.3101
2390 520.2576 519.2503
2391 524.2853 523.2780
2392 524.2853 523.2780
2393 524.2860 523.2787
2394 527.1824 526.1751
2395 528.2502 527.2429
2396 528.7712 527.7639
2397 529.2744 528.2671
2399 529.2756 528.2683
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