MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2196 439.1969 438.1896
2197 439.1979 438.1906
2198 439.2134 438.2061
2199 439.2143 438.2070
2200 439.2144 438.2071
2201 439.2149 438.2076
2202 439.2150 438.2077
2203 439.2150 438.2077
2204 439.2153 438.2080
2205 439.2155 438.2082
2206 439.2157 438.2084
2207 439.2159 438.2086
2208 439.2160 438.2087
2209 439.2310 438.2237
2210 439.2317 438.2244
2211 439.2317 438.2244
2212 439.2318 438.2245
2213 439.2327 438.2254
2214 439.2331 438.2258
2215 439.2331 438.2258
2216 439.2334 438.2261
2217 439.2336 438.2263
2218 439.2338 438.2265
2219 439.2349 438.2276
2220 439.2366 438.2293
2221 441.1102 440.1029
2222 441.1464 440.1391
2223 441.2104 440.2031
2224 441.2115 440.2042
2225 441.2307 440.2234
2226 441.2311 440.2238
2227 441.2318 440.2245
2228 442.2430 441.2357
2229 442.2435 441.2362
2230 443.0436 442.0363
2231 443.1246 442.1173
2232 443.1255 442.1182
2233 443.2463 442.2390
2234 443.2469 442.2396
2235 443.2469 442.2396
2236 443.2469 442.2396
2237 443.2478 442.2405
2238 443.2498 442.2425
2239 444.1108 443.1035
2240 444.4796 443.4723
2241 445.1415 444.1342
2242 445.2248 444.2175
2243 445.2260 444.2187
2244 448.2360 447.2287
2245 448.2362 447.2289
2246 448.3123 447.3050
2247 448.3130 447.3057
2248 449.1524 448.1451
2249 449.2167 448.2094
2250 449.2179 448.2106
2251 449.2180 448.2107
2252 449.2552 448.2479
2253 450.5201 449.5128
2254 451.1683 450.1610
2255 451.1686 450.1613
2256 451.1940 450.1867
2257 451.1954 450.1881
2258 451.1958 450.1885
2259 451.1967 450.1894
2260 451.1972 450.1899
2261 451.1973 450.1900
2262 451.1979 450.1906
2263 451.2332 450.2259
2264 451.2333 450.2260
2265 451.2334 450.2261
2266 451.2341 450.2268
2267 451.2342 450.2269
2268 451.2347 450.2274
2269 451.2352 450.2279
2270 453.2107 452.2034
2271 453.2110 452.2037
2272 453.2118 452.2045
2273 453.2119 452.2046
2274 453.2120 452.2047
2275 453.2121 452.2048
2276 453.2125 452.2052
2277 453.2125 452.2052
2278 453.2126 452.2053
2279 453.2127 452.2054
2280 453.2128 452.2055
2281 453.2473 452.2400
2282 453.2481 452.2408
2283 453.2484 452.2411
2284 453.2589 452.2516
2285 453.2595 452.2522
2286 453.3450 452.3377
2287 455.1614 454.1541
2288 455.1913 454.1840
2289 455.2064 454.1991
2290 455.2266 454.2193
2291 455.2273 454.2200
2292 455.2283 454.2210
2293 455.2286 454.2213
2294 455.2286 454.2213
2295 457.1405 456.1332
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