MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2096 425.1808 424.1735
2097 425.1808 424.1735
2098 425.2161 424.2088
2099 425.2165 424.2092
2100 425.2167 424.2094
2101 425.2168 424.2095
2102 425.2169 424.2096
2103 425.2169 424.2096
2104 425.2171 424.2098
2105 425.2171 424.2098
2106 425.2172 424.2099
2107 425.2172 424.2099
2108 425.2175 424.2102
2109 425.2178 424.2105
2110 425.2180 424.2107
2111 425.2180 424.2107
2112 425.2191 424.2118
2113 425.2343 424.2270
2114 425.2345 424.2272
2115 425.2355 424.2282
2116 425.2358 424.2285
2117 425.2359 424.2286
2118 425.2359 424.2286
2119 425.2361 424.2288
2120 425.2362 424.2289
2121 425.2362 424.2289
2122 425.2368 424.2295
2123 425.2637 424.2564
2124 427.0944 426.0871
2125 427.1285 426.1212
2126 427.1317 426.1244
2127 427.1653 426.1580
2128 427.1963 426.1890
2129 427.1964 426.1891
2130 427.1972 426.1899
2131 427.2313 426.2240
2132 427.2334 426.2261
2133 427.2568 426.2495
2134 428.2554 427.2481
2135 429.0906 428.0833
2136 429.1088 428.1015
2137 429.1115 428.1042
2138 429.1477 428.1404
2139 429.2677 428.2604
2140 430.2797 429.2724
2141 430.2798 429.2725
2142 430.3014 429.2941
2143 430.3015 429.2942
2144 430.3019 429.2946
2145 431.1260 430.1187
2146 431.2095 430.2022
2147 433.1858 432.1785
2148 433.1867 432.1794
2149 433.2226 432.2153
2150 435.1270 434.1197
2151 435.2008 434.1935
2152 435.2009 434.1936
2153 435.2010 434.1937
2154 435.2016 434.1943
2155 435.2018 434.1945
2156 435.2018 434.1945
2157 435.2018 434.1945
2158 435.2019 434.1946
2159 435.2020 434.1947
2160 435.2022 434.1949
2161 435.2022 434.1949
2162 435.2023 434.1950
2163 435.2023 434.1950
2164 435.2385 434.2312
2165 435.2392 434.2319
2166 435.2393 434.2320
2167 435.2397 434.2324
2168 435.2416 434.2343
2169 437.1803 436.1730
2170 437.1805 436.1732
2171 437.1811 436.1738
2172 437.1814 436.1741
2173 437.1816 436.1743
2174 437.2152 436.2079
2175 437.2153 436.2080
2176 437.2163 436.2090
2177 437.2166 436.2093
2178 437.2169 436.2096
2179 437.2170 436.2097
2180 437.2174 436.2101
2181 437.2175 436.2102
2182 437.2175 436.2102
2183 437.2175 436.2102
2184 437.2176 436.2103
2185 437.2179 436.2106
2186 437.2180 436.2107
2187 437.2180 436.2107
2188 437.2382 436.2309
2189 437.2530 436.2457
2190 437.2533 436.2460
2191 439.1953 438.1880
2192 439.1956 438.1883
2193 439.1959 438.1886
2194 439.1967 438.1894
2195 439.1967 438.1894
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