MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1996 416.3764 415.3691
1997 417.2467 416.2394
1998 417.2474 416.2401
1999 417.2485 416.2412
2000 417.2487 416.2414
2001 419.1704 418.1631
2002 419.1963 418.1890
2003 419.2054 418.1981
2004 419.2061 418.1988
2005 419.2065 418.1992
2006 419.2067 418.1994
2007 419.2070 418.1997
2008 419.2072 418.1999
2009 419.2074 418.2001
2010 419.2075 418.2002
2011 419.2086 418.2013
2012 419.2440 418.2367
2013 419.2441 418.2368
2014 421.0588 420.0515
2015 421.1478 420.1405
2016 421.1846 420.1773
2017 421.1848 420.1775
2018 421.1851 420.1778
2019 421.1852 420.1779
2020 421.1854 420.1781
2021 421.1854 420.1781
2022 421.1862 420.1789
2023 421.1862 420.1789
2024 421.1863 420.1790
2025 421.1869 420.1796
2026 421.1871 420.1798
2027 421.2029 420.1956
2028 421.2034 420.1961
2029 421.2041 420.1968
2030 421.2045 420.1972
2031 421.2047 420.1974
2032 421.2047 420.1974
2033 421.2047 420.1974
2034 421.2049 420.1976
2035 421.2052 420.1979
2036 421.2209 420.2136
2037 421.2215 420.2142
2038 421.2216 420.2143
2039 421.2227 420.2154
2040 421.2227 420.2154
2041 421.2228 420.2155
2042 421.2228 420.2155
2043 421.2230 420.2157
2044 421.2231 420.2158
2045 421.2231 420.2158
2046 421.2234 420.2161
2047 423.0740 422.0667
2048 423.1356 422.1283
2049 423.1636 422.1563
2050 423.1638 422.1565
2051 423.1654 422.1581
2052 423.1654 422.1581
2053 423.1656 422.1583
2054 423.1997 422.1924
2055 423.1999 422.1926
2056 423.2004 422.1931
2057 423.2006 422.1933
2058 423.2007 422.1934
2059 423.2007 422.1934
2060 423.2009 422.1936
2061 423.2009 422.1936
2062 423.2009 422.1936
2063 423.2011 422.1938
2064 423.2011 422.1938
2065 423.2012 422.1939
2066 423.2012 422.1939
2067 423.2015 422.1942
2068 423.2017 422.1944
2069 423.2018 422.1945
2070 423.2019 422.1946
2071 423.2021 422.1948
2072 423.2021 422.1948
2073 423.2023 422.1950
2074 423.2183 422.2110
2075 423.2188 422.2115
2076 423.2193 422.2120
2077 423.2199 422.2126
2078 423.2202 422.2129
2079 423.2203 422.2130
2080 423.2206 422.2133
2081 423.2209 422.2136
2082 423.2263 422.2190
2083 423.2272 422.2199
2084 423.2386 422.2313
2085 423.2387 422.2314
2086 423.2388 422.2315
2087 425.1517 424.1444
2088 425.1792 424.1719
2089 425.1793 424.1720
2090 425.1796 424.1723
2091 425.1800 424.1727
2092 425.1801 424.1728
2093 425.1804 424.1731
2094 425.1804 424.1731
2095 425.1805 424.1732
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