MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1896 407.2086 406.2013
1897 407.2236 406.2163
1898 407.2241 406.2168
1899 407.2247 406.2174
1900 407.2249 406.2176
1901 407.2250 406.2177
1902 407.2252 406.2179
1903 407.2253 406.2180
1904 407.2256 406.2183
1905 407.2256 406.2183
1906 407.2261 406.2188
1907 407.2430 406.2357
1908 409.0847 408.0774
1909 409.1479 408.1406
1910 409.1492 408.1419
1911 409.1496 408.1423
1912 409.1500 408.1427
1913 409.1647 408.1574
1914 409.1841 408.1768
1915 409.1848 408.1775
1916 409.1848 408.1775
1917 409.1848 408.1775
1918 409.1850 408.1777
1919 409.1853 408.1780
1920 409.1854 408.1781
1921 409.1854 408.1781
1922 409.1859 408.1786
1923 409.1861 408.1788
1924 409.1861 408.1788
1925 409.1861 408.1788
1926 409.1862 408.1789
1927 409.1863 408.1790
1928 409.1865 408.1792
1929 409.1865 408.1792
1930 409.2213 408.2140
1931 409.2215 408.2142
1932 409.2218 408.2145
1933 409.2223 408.2150
1934 409.2225 408.2152
1935 409.2228 408.2155
1936 409.2231 408.2158
1937 409.2231 408.2158
1938 409.2390 408.2317
1939 409.2398 408.2325
1940 409.2408 408.2335
1941 409.2409 408.2336
1942 409.2409 408.2336
1943 409.2411 408.2338
1944 410.2437 409.2364
1945 410.2438 409.2365
1946 410.2444 409.2371
1947 410.2449 409.2376
1948 411.0720 410.0647
1949 411.0997 410.0924
1950 411.1334 410.1261
1951 411.1625 410.1552
1952 411.1635 410.1562
1953 411.1643 410.1570
1954 411.1647 410.1574
1955 411.1648 410.1575
1956 411.1648 410.1575
1957 411.1655 410.1582
1958 411.1659 410.1586
1959 411.2003 410.1930
1960 411.2004 410.1931
1961 411.2005 410.1932
1962 411.2007 410.1934
1963 411.2008 410.1935
1964 411.2008 410.1935
1965 411.2013 410.1940
1966 411.2015 410.1942
1967 411.2016 410.1943
1968 411.2019 410.1946
1969 411.2024 410.1951
1970 412.2299 411.2226
1971 413.1146 412.1073
1972 413.1152 412.1079
1973 413.1517 412.1444
1974 413.1802 412.1729
1975 413.1808 412.1735
1976 413.1811 412.1738
1977 413.1814 412.1741
1978 413.1817 412.1744
1979 413.2679 412.2606
1980 413.2687 412.2614
1981 413.2727 412.2654
1982 414.2476 413.2403
1983 415.0935 414.0862
1984 415.0946 414.0873
1985 415.1306 414.1233
1986 415.1318 414.1245
1987 415.1659 414.1586
1988 415.1964 414.1891
1989 416.2623 415.2550
1990 416.2629 415.2556
1991 416.2629 415.2556
1992 416.2639 415.2566
1993 416.2647 415.2574
1994 416.3757 415.3684
1995 416.3759 415.3686
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