MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1796 395.2067 394.1994
1797 395.2072 394.1999
1798 395.2256 394.2183
1799 395.2640 394.2567
1800 397.0833 396.0760
1801 397.1196 396.1123
1802 397.1495 396.1422
1803 397.1496 396.1423
1804 397.1497 396.1424
1805 397.1502 396.1429
1806 397.1840 396.1767
1807 397.1841 396.1768
1808 397.1846 396.1773
1809 397.1848 396.1775
1810 397.1851 396.1778
1811 397.1853 396.1780
1812 397.1857 396.1784
1813 397.1857 396.1784
1814 397.1859 396.1786
1815 397.1859 396.1786
1816 397.1859 396.1786
1817 397.1860 396.1787
1818 397.1860 396.1787
1819 397.1861 396.1788
1820 397.1862 396.1789
1821 397.1864 396.1791
1822 397.1866 396.1793
1823 397.2206 396.2133
1824 397.2211 396.2138
1825 397.2216 396.2143
1826 397.2829 396.2756
1827 399.0995 398.0922
1828 399.0999 398.0926
1829 399.1522 398.1449
1830 399.2007 398.1934
1831 399.2008 398.1935
1832 399.2010 398.1937
1833 399.2010 398.1937
1834 399.2018 398.1945
1835 399.2018 398.1945
1836 399.2019 398.1946
1837 399.2215 398.2142
1838 399.2354 398.2281
1839 401.0777 400.0704
1840 401.1146 400.1073
1841 401.1152 400.1079
1842 401.1154 400.1081
1843 401.2363 400.2290
1844 401.2882 400.2809
1845 401.2884 400.2811
1846 402.2477 401.2404
1847 402.2482 401.2409
1848 403.1759 402.1686
1849 403.2329 402.2256
1850 404.2058 403.1985
1851 405.1903 404.1830
1852 405.1905 404.1832
1853 405.1907 404.1834
1854 405.1908 404.1835
1855 405.1910 404.1837
1856 405.1911 404.1838
1857 405.1913 404.1840
1858 405.1913 404.1840
1859 405.1914 404.1841
1860 405.1915 404.1842
1861 405.1915 404.1842
1862 405.1916 404.1843
1863 405.2101 404.2028
1864 405.2156 404.2083
1865 405.2270 404.2197
1866 405.2270 404.2197
1867 405.2280 404.2207
1868 405.2285 404.2212
1869 407.1317 406.1244
1870 407.1320 406.1247
1871 407.1688 406.1615
1872 407.1691 406.1618
1873 407.1691 406.1618
1874 407.1693 406.1620
1875 407.1694 406.1621
1876 407.1694 406.1621
1877 407.1696 406.1623
1878 407.1696 406.1623
1879 407.1703 406.1630
1880 407.1706 406.1633
1881 407.1706 406.1633
1882 407.2054 406.1981
1883 407.2057 406.1984
1884 407.2061 406.1988
1885 407.2062 406.1989
1886 407.2063 406.1990
1887 407.2066 406.1993
1888 407.2066 406.1993
1889 407.2067 406.1994
1890 407.2068 406.1995
1891 407.2070 406.1997
1892 407.2071 406.1998
1893 407.2074 406.2001
1894 407.2078 406.2005
1895 407.2079 406.2006
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