MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1696 393.0893 392.0820
1697 393.1522 392.1449
1698 393.1527 392.1454
1699 393.1529 392.1456
1700 393.1531 392.1458
1701 393.1532 392.1459
1702 393.1537 392.1464
1703 393.1538 392.1465
1704 393.1538 392.1465
1705 393.1538 392.1465
1706 393.1544 392.1471
1707 393.1546 392.1473
1708 393.1550 392.1477
1709 393.1895 392.1822
1710 393.1897 392.1824
1711 393.1899 392.1826
1712 393.1900 392.1827
1713 393.1901 392.1828
1714 393.1902 392.1829
1715 393.1903 392.1830
1716 393.1905 392.1832
1717 393.1906 392.1833
1718 393.1907 392.1834
1719 393.1908 392.1835
1720 393.1911 392.1838
1721 393.1911 392.1838
1722 393.1913 392.1840
1723 393.1913 392.1840
1724 393.1916 392.1843
1725 393.1916 392.1843
1726 393.2104 392.2031
1727 393.2119 392.2046
1728 393.2284 392.2211
1729 393.2468 392.2395
1730 393.2469 392.2396
1731 393.2495 392.2422
1732 393.2986 392.2913
1733 393.2987 392.2914
1734 393.2987 392.2914
1735 393.2987 392.2914
1736 393.2987 392.2914
1737 393.2988 392.2915
1738 393.2990 392.2917
1739 393.2990 392.2917
1740 393.2990 392.2917
1741 393.2991 392.2918
1742 393.2991 392.2918
1743 393.2991 392.2918
1744 393.2992 392.2919
1745 393.2992 392.2919
1746 393.2994 392.2921
1747 393.2995 392.2922
1748 393.2996 392.2923
1749 393.2996 392.2923
1750 393.2996 392.2923
1751 393.2997 392.2924
1752 393.2998 392.2925
1753 393.2998 392.2925
1754 393.3000 392.2927
1755 393.3000 392.2927
1756 393.3000 392.2927
1757 393.3000 392.2927
1758 393.3001 392.2928
1759 393.3002 392.2929
1760 393.3003 392.2930
1761 393.3003 392.2930
1762 393.3003 392.2930
1763 393.3004 392.2931
1764 393.3004 392.2931
1765 393.3006 392.2933
1766 393.3007 392.2934
1767 395.0768 394.0695
1768 395.0776 394.0703
1769 395.1687 394.1614
1770 395.1689 394.1616
1771 395.1691 394.1618
1772 395.1692 394.1619
1773 395.1693 394.1620
1774 395.1695 394.1622
1775 395.1695 394.1622
1776 395.1696 394.1623
1777 395.1697 394.1624
1778 395.1698 394.1625
1779 395.1700 394.1627
1780 395.1704 394.1631
1781 395.1704 394.1631
1782 395.1705 394.1632
1783 395.1705 394.1632
1784 395.1707 394.1634
1785 395.1707 394.1634
1786 395.1707 394.1634
1787 395.2050 394.1977
1788 395.2054 394.1981
1789 395.2054 394.1981
1790 395.2057 394.1984
1791 395.2063 394.1990
1792 395.2063 394.1990
1793 395.2066 394.1993
1794 395.2066 394.1993
1795 395.2067 394.1994
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