MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1596 381.2107 380.2034
1597 383.1031 382.0958
1598 383.1031 382.0958
1599 383.1316 382.1243
1600 383.1337 382.1264
1601 383.1344 382.1271
1602 383.1685 382.1612
1603 383.1688 382.1615
1604 383.1691 382.1618
1605 383.1691 382.1618
1606 383.1692 382.1619
1607 383.1693 382.1620
1608 383.1693 382.1620
1609 383.1695 382.1622
1610 383.1698 382.1625
1611 383.1701 382.1628
1612 383.1701 382.1628
1613 383.1703 382.1630
1614 383.1703 382.1630
1615 383.1711 382.1638
1616 383.2053 382.1980
1617 383.2055 382.1982
1618 383.2057 382.1984
1619 383.2066 382.1993
1620 383.2068 382.1995
1621 383.2069 382.1996
1622 383.2070 382.1997
1623 383.2070 382.1997
1624 383.2073 382.2000
1625 383.2075 382.2002
1626 383.2262 382.2189
1627 383.2264 382.2191
1628 383.2270 382.2197
1629 385.0831 384.0758
1630 385.0832 384.0759
1631 385.1190 384.1117
1632 385.1201 384.1128
1633 385.1364 384.1291
1634 385.1481 384.1408
1635 385.1567 384.1494
1636 385.1839 384.1766
1637 385.1842 384.1769
1638 385.1848 384.1775
1639 385.1852 384.1779
1640 385.1857 384.1784
1641 385.1858 384.1785
1642 385.1859 384.1786
1643 385.1861 384.1788
1644 385.1862 384.1789
1645 385.1862 384.1789
1646 385.1863 384.1790
1647 385.1863 384.1790
1648 385.2024 384.1951
1649 385.2036 384.1963
1650 385.2217 384.2144
1651 385.2567 384.2494
1652 385.2572 384.2499
1653 385.2578 384.2505
1654 385.2580 384.2507
1655 386.2408 385.2335
1656 387.0980 386.0907
1657 387.0989 386.0916
1658 387.1359 386.1286
1659 387.1999 386.1926
1660 387.2002 386.1929
1661 387.2003 386.1930
1662 387.2003 386.1930
1663 387.2017 386.1944
1664 387.2018 386.1945
1665 387.2018 386.1945
1666 387.2043 386.1970
1667 387.2378 386.2305
1668 387.2387 386.2314
1669 388.2326 387.2253
1670 389.0527 388.0454
1671 389.1508 388.1435
1672 389.1597 388.1524
1673 389.1964 388.1891
1674 389.1966 388.1893
1675 389.1972 388.1899
1676 389.1972 388.1899
1677 391.1740 390.1667
1678 391.1744 390.1671
1679 391.1745 390.1672
1680 391.1746 390.1673
1681 391.1751 390.1678
1682 391.1754 390.1681
1683 391.1757 390.1684
1684 391.1758 390.1685
1685 391.1759 390.1686
1686 391.2111 390.2038
1687 391.2119 390.2046
1688 391.2123 390.2050
1689 391.2124 390.2051
1690 391.2124 390.2051
1691 391.2126 390.2053
1692 391.2128 390.2055
1693 391.2308 390.2235
1694 391.2863 390.2790
1695 391.2865 390.2792
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