MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1496 377.1581 376.1508
1497 377.1584 376.1511
1498 377.1585 376.1512
1499 377.1588 376.1515
1500 377.1588 376.1515
1501 377.1588 376.1515
1502 377.1589 376.1516
1503 377.1594 376.1521
1504 377.1595 376.1522
1505 377.1598 376.1525
1506 377.1598 376.1525
1507 377.1601 376.1528
1508 377.1603 376.1530
1509 377.1950 376.1877
1510 377.1956 376.1883
1511 377.1960 376.1887
1512 377.1960 376.1887
1513 377.1962 376.1889
1514 377.1963 376.1890
1515 377.1963 376.1890
1516 377.1964 376.1891
1517 377.1964 376.1891
1518 377.1966 376.1893
1519 377.1967 376.1894
1520 378.0260 377.0187
1521 379.0999 378.0926
1522 379.1380 378.1307
1523 379.1381 378.1308
1524 379.1381 378.1308
1525 379.1386 378.1313
1526 379.1389 378.1316
1527 379.1390 378.1317
1528 379.1394 378.1321
1529 379.1737 378.1664
1530 379.1738 378.1665
1531 379.1738 378.1665
1532 379.1741 378.1668
1533 379.1742 378.1669
1534 379.1744 378.1671
1535 379.1744 378.1671
1536 379.1744 378.1671
1537 379.1745 378.1672
1538 379.1749 378.1676
1539 379.1752 378.1679
1540 379.1752 378.1679
1541 379.1752 378.1679
1542 379.1753 378.1680
1543 379.1753 378.1680
1544 379.1754 378.1681
1545 379.1754 378.1681
1546 379.1756 378.1683
1547 379.1758 378.1685
1548 379.1758 378.1685
1549 379.1944 378.1871
1550 379.2102 378.2029
1551 379.2113 378.2040
1552 379.2115 378.2042
1553 379.2116 378.2043
1554 379.2116 378.2043
1555 379.2120 378.2047
1556 379.2122 378.2049
1557 379.2123 378.2050
1558 379.2126 378.2053
1559 381.1529 380.1456
1560 381.1530 380.1457
1561 381.1535 380.1462
1562 381.1538 380.1465
1563 381.1539 380.1466
1564 381.1541 380.1468
1565 381.1541 380.1468
1566 381.1542 380.1469
1567 381.1542 380.1469
1568 381.1543 380.1470
1569 381.1546 380.1473
1570 381.1548 380.1475
1571 381.1548 380.1475
1572 381.1890 380.1817
1573 381.1893 380.1820
1574 381.1894 380.1821
1575 381.1897 380.1824
1576 381.1897 380.1824
1577 381.1898 380.1825
1578 381.1899 380.1826
1579 381.1900 380.1827
1580 381.1901 380.1828
1581 381.1905 380.1832
1582 381.1906 380.1833
1583 381.1907 380.1834
1584 381.1908 380.1835
1585 381.1908 380.1835
1586 381.1908 380.1835
1587 381.1909 380.1836
1588 381.1909 380.1836
1589 381.1910 380.1837
1590 381.1912 380.1839
1591 381.1912 380.1839
1592 381.1915 380.1842
1593 381.1918 380.1845
1594 381.2105 380.2032
1595 381.2106 380.2033
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