MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1396 369.1908 368.1835
1397 369.1910 368.1837
1398 369.1910 368.1837
1399 369.1911 368.1838
1400 369.1912 368.1839
1401 369.1912 368.1839
1402 369.2094 368.2021
1403 369.2095 368.2022
1404 369.2118 368.2045
1405 369.2258 368.2185
1406 369.2264 368.2191
1407 369.2266 368.2193
1408 369.2273 368.2200
1409 369.2274 368.2201
1410 369.2274 368.2201
1411 369.2275 368.2202
1412 369.2276 368.2203
1413 369.2277 368.2204
1414 369.2280 368.2207
1415 369.2479 368.2406
1416 369.2481 368.2408
1417 369.2481 368.2408
1418 369.2484 368.2411
1419 371.0650 370.0577
1420 371.1031 370.0958
1421 371.1034 370.0961
1422 371.1035 370.0962
1423 371.1039 370.0966
1424 371.1206 370.1133
1425 371.1391 370.1318
1426 371.1573 370.1500
1427 371.1694 370.1621
1428 371.1709 370.1636
1429 371.2413 370.2340
1430 371.2423 370.2350
1431 371.3165 370.3092
1432 371.3166 370.3093
1433 371.3167 370.3094
1434 371.3168 370.3095
1435 371.3168 370.3095
1436 371.3169 370.3096
1437 371.3170 370.3097
1438 371.3170 370.3097
1439 371.3170 370.3097
1440 371.3171 370.3098
1441 371.3171 370.3098
1442 371.3172 370.3099
1443 371.3172 370.3099
1444 371.3172 370.3099
1445 371.3174 370.3101
1446 371.3175 370.3102
1447 371.3175 370.3102
1448 371.3176 370.3103
1449 371.3177 370.3104
1450 371.3178 370.3105
1451 371.3179 370.3106
1452 371.3179 370.3106
1453 371.3179 370.3106
1454 371.3180 370.3107
1455 371.3180 370.3107
1456 371.3180 370.3107
1457 371.3181 370.3108
1458 371.3181 370.3108
1459 371.3182 370.3109
1460 371.3182 370.3109
1461 371.3183 370.3110
1462 371.3183 370.3110
1463 371.3183 370.3110
1464 371.3184 370.3111
1465 371.3188 370.3115
1466 372.0525 371.0452
1467 372.1941 371.1868
1468 372.1942 371.1869
1469 373.0828 372.0755
1470 373.1201 372.1128
1471 373.2213 372.2140
1472 373.2223 372.2150
1473 374.2927 373.2854
1474 374.2928 373.2855
1475 374.2932 373.2859
1476 375.1062 374.0989
1477 375.1065 374.0992
1478 375.1436 374.1363
1479 375.1807 374.1734
1480 375.1807 374.1734
1481 375.1812 374.1739
1482 375.1812 374.1739
1483 375.1997 374.1924
1484 375.2000 374.1927
1485 375.2005 374.1932
1486 375.2007 374.1934
1487 375.2011 374.1938
1488 375.2170 374.2097
1489 375.2176 374.2103
1490 375.2364 374.2291
1491 375.2368 374.2295
1492 376.2335 375.2262
1493 376.2354 375.2281
1494 377.1212 376.1139
1495 377.1213 376.1140
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