MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2647 727.3770 726.3697 726.3773 -10.4 0 8 0.18 +1Score > 13 indicates identity SSLHGAR
2754 893.4478 892.4405 892.4403 0.29 1 8 0.5 +1Score > 18 indicates identity AAFEDKGR
2808 465.2644 928.5142 928.5090 5.65 1 8 0.58 +1Score > 18 indicates identity RLEQIDR
3104 572.3235 1142.6324 1142.6295 2.58 0 8 0.45 +1Score > 17 indicates identity VNLIASQIQR + 2 Deamidated (NQ)
3588 731.3749 1460.7352 1460.7623 -18.5 1 8 0.85 +1Score > 20 indicates identity IENKLANEGFVAR + Deamidated (NQ)
4076 670.6828 2009.0266 2009.0079 9.31 1 8 0.71 +1Score > 19 indicates identity RWLESQGVDVANGSNHLK
2744 442.7468 883.4790 883.4763 3.12 0 8 0.27 +1Score > 15 indicates identity EALNLPAR + Deamidated (NQ)
3203 627.3008 1252.5870 1252.5692 14.2 0 8 0.47 +1Score > 17 indicates identity SNHYGMIIMR + 2 Oxidation (M)
3148 601.8011 1201.5876 1201.5938 -5.14 1 8 0.73 +1Score > 19 indicates identity AEQAALQADKR + 2 Deamidated (NQ)
2570 338.6754 675.3362 675.3374 -1.66 0 8 0.68 +1Score > 19 indicates identity GMLEAR
2404 529.2793 528.2720 528.2656 12.2 0 8 0.16 +1Score > 13 indicates identity PANAR + Deamidated (NQ)
3517 710.8474 1419.6802 1419.6630 12.2 0 8 0.67 +1Score > 19 indicates identity SLTWNGITQQNR + 3 Deamidated (NQ)
2564 672.4080 671.4007 671.3966 6.13 0 8 0.83 +1Score > 20 indicates identity GNVLIR + Deamidated (NQ)
2954 338.1614 1011.4624 1011.4444 17.8 0 8 0.29 +1Score > 15 indicates identity MTPSNYQR + Oxidation (M)
3616 372.6673 1486.6401 1486.6609 -14.0 0 8 0.3 +1Score > 15 indicates identity GMLNELDEEPSPR + Deamidated (NQ)
4245 610.8032 2439.1837 2439.2104 -11.0 0 8 0.92 +1Score > 20 indicates identity FVQPGTQVYDLGCSLGAATLSVR + Deamidated (NQ)
3448 345.9420 1379.7389 1379.7483 -6.80 0 8 0.37 +1Score > 16 indicates identity IIFCGTLTAGSLK
2723 428.7505 855.4864 855.4715 17.5 0 8 0.3 +1Score > 15 indicates identity RPLWQR + Deamidated (NQ)
3034 533.7429 1065.4712 1065.4727 -1.34 0 8 0.3 +1Score > 15 indicates identity GQGAQLNGYR + 3 Deamidated (NQ)
2678 386.7214 771.4282 771.4391 -14.1 0 8 0.68 +1Score > 18 indicates identity LAIYHR
4244 488.8431 2439.1791 2439.2250 -18.8 1 8 0.91 +1Score > 20 indicates identity SNIGHPQGAAGVAGVIKMVMALQR + 3 Deamidated (NQ); 2 Oxidation (M)
2637 720.4064 719.3991 719.3966 3.48 0 8 0.5 +1Score > 17 indicates identity FDALVR
3053 540.7383 1079.4620 1079.4739 -11.0 0 7 0.26 +1Score > 14 indicates identity EDMSMQAIR
3154 603.3068 1204.5990 1204.5870 9.98 1 7 0.77 +1Score > 20 indicates identity
Score > 19 indicates homology		 DRDLIGMVDR + Oxidation (M)
2910 494.7590 987.5034 987.5171 -13.8 0 7 0.88 +1Score > 19 indicates identity LAELMAANR
2809 930.5289 929.5216 929.5182 3.71 0 7 0.68 +1Score > 18 indicates identity IITQADLR + Deamidated (NQ)
3167 307.3943 1225.5481 1225.5397 6.84 0 7 0.31 +1Score > 15 indicates identity MEQYDQIGAR + Oxidation (M)
3347 669.3583 1336.7020 1336.7099 -5.85 1 7 0.72 +1Score > 18 indicates identity SPSAQKLEALHR + Deamidated (NQ)
3674 555.9612 1664.8618 1664.8774 -9.36 1 7 0.72 +1Score > 18 indicates identity AQVAIFKEIFDQVR + 2 Deamidated (NQ)
3095 562.7937 1123.5728 1123.5734 -0.49 1 7 0.64 +1Score > 18 indicates identity QAQELHDKR
2650 365.2353 728.4560 728.4545 2.19 0 7 0.68 +1Score > 18 indicates identity SVAIALR
3105 572.7747 1143.5348 1143.5421 -6.34 1 7 0.39 +1Score > 15 indicates identity HTTRNFPNR + 2 Deamidated (NQ)
3198 624.3000 1246.5854 1246.5830 1.97 0 7 0.71 +1Score > 18 indicates identity DDIFSVPSPDR
3498 702.8515 1403.6884 1403.6980 -6.77 1 7 0.87 +1Score > 19 indicates identity RLMTASWPGNVR + Deamidated (NQ); Oxidation (M)
3451 345.9427 1379.7417 1379.7483 -4.77 0 7 0.44 +1Score > 16 indicates identity IIFCGTLTAGSLK
2589 346.6735 691.3324 691.3323 0.23 0 7 0.78 +1Score > 18 indicates identity MAGLER + Oxidation (M)
2973 513.7328 1025.4510 1025.4600 -8.74 0 7 0.35 +1Score > 15 indicates identity ISQYACQR + Deamidated (NQ)
4107 677.6769 2030.0089 2030.0221 -6.52 1 7 1 +1Score > 19 indicates identity RDWQLQQLGITQWSLR + 3 Deamidated (NQ)
2861 479.2629 956.5112 956.5291 -18.6 0 7 0.94 +1Score > 19 indicates identity QDILEAIR
3756 438.9861 1751.9153 1751.8916 13.5 0 7 0.69 +1Score > 18 indicates identity LVPGYEAPVMLAYSAR + Oxidation (M)
3461 694.8694 1387.7242 1387.7030 15.3 1 7 0.92 +1Score > 19 indicates identity RLMTASWPGNVR + Deamidated (NQ)
3567 721.8608 1441.7070 1441.7201 -9.07 0 7 0.5 +1Score > 16 indicates identity FHAQPLSANDTLK + Deamidated (NQ)
3268 643.3051 1284.5956 1284.5955 0.15 0 7 0.48 +1Score > 16 indicates identity MVTNLYQNMR + Oxidation (M)
3166 613.2924 1224.5702 1224.5809 -8.68 0 7 0.55 +1Score > 17 indicates identity GVFNMLLSDGR + Deamidated (NQ); Oxidation (M)
2712 418.7062 835.3978 835.3970 0.99 1 7 0.69 +1Score > 17 indicates identity GRMESTR
3235 423.5175 1267.5307 1267.5537 -18.1 1 7 0.33 +1Score > 14 indicates identity DAAMVADMREK + 2 Oxidation (M)
2958 507.7693 1013.5240 1013.5182 5.78 0 7 0.72 +1Score > 18 indicates identity GTYFLGSAAK
3149 601.8015 1201.5884 1201.5938 -4.48 1 7 1.1 +1Score > 19 indicates identity AEQAALQADKR + 2 Deamidated (NQ)
3772 440.2390 1756.9269 1756.9472 -11.5 1 7 0.86 +1Score > 18 indicates identity LTDSFRGISVIPAEPR
4073 1005.0219 2008.0292 2008.0126 8.28 1 7 0.89 +1Score > 19 indicates identity HRIQVVFQDPNSSLNPR + 2 Deamidated (NQ)
3667 546.9741 1637.9005 1637.8736 16.4 0 6 0.4 +1Score > 15 indicates identity ALGAKPQINAEEEIR
2654 732.3300 731.3227 731.3198 3.99 0 6 0.23 +1Score > 13 indicates identity NQEGQR + Deamidated (NQ)
2688 397.2400 792.4654 792.4606 6.12 1 6 0.25 +1Score > 13 indicates identity SLRVYR
3298 654.3102 1306.6058 1306.6261 -15.5 0 6 0.61 +1Score > 17 indicates identity ELALLMSDAGCK
2521 324.1742 646.3338 646.3221 18.2 0 6 0.94 +1Score > 19 indicates identity AAACVR
4227 806.7575 2417.2507 2417.2173 13.8 0 6 0.69 +1Score > 17 indicates identity LQTLGLTQGTVVTISAEGEDEQK + Deamidated (NQ)
2576 684.3632 683.3559 683.3602 -6.28 0 6 0.34 +1Score > 14 indicates identity IPSNPR + Deamidated (NQ)
3927 471.4743 1881.8681 1881.8719 -2.04 1 6 0.98 +1Score > 19 indicates identity FAFQNARAFEYLMTR + 2 Deamidated (NQ); Oxidation (M)
3259 640.3384 1278.6622 1278.6390 18.2 1 6 1 +1Score > 19 indicates identity KAFQDMEAALR
3161 610.3028 1218.5910 1218.6092 -14.9 0 6 1.1 +1Score > 19 indicates identity DTVSELLTANR + Deamidated (NQ)
2496 315.1700 628.3254 628.3367 -17.8 0 6 0.58 +1Score > 16 indicates identity HMTIK
4125 409.4007 2041.9671 2042.0004 -16.3 1 6 0.5 +1Score > 20 indicates identity
Score > 16 indicates homology		 HTNWQVCSLVVQAKSER + Deamidated (NQ)
2841 317.8344 950.4814 950.4855 -4.35 1 6 0.84 +1Score > 18 indicates identity RMLVSSDK + Oxidation (M)
3797 593.5760 1777.7062 1777.6962 5.62 0 6 0.25 +1Score > 13 indicates identity GNGENSYGGNGDMTYAR + Oxidation (M)
4356 654.0585 2612.2049 2612.2329 -10.7 1 6 0.95 +1Score > 18 indicates identity IWDFNGGIHPPEMKTQSNGTPLR + 2 Deamidated (NQ); Oxidation (M)
2939 502.2814 1002.5482 1002.5345 13.7 1 6 0.96 +1Score > 18 indicates identity IQSIEEKR + Deamidated (NQ)
4587 949.2273 5689.3201 5689.2571 11.1 1 6 0.26 +1Score > 13 indicates identity HSAEIANPLWFFLIVITLFPLSIGPEPQLLARIAPGIIWVAALLSSLLALER
2398 529.2751 528.2678 528.2656 4.22 0 6 0.27 +1Score > 13 indicates identity AGPQR + Deamidated (NQ)
3040 535.7625 1069.5104 1069.5001 9.65 0 6 0.53 +1Score > 16 indicates identity LYELEMQK + Deamidated (NQ); Oxidation (M)
3291 651.3010 1300.5874 1300.6122 -19.0 0 6 0.51 +1Score > 15 indicates identity MFNTAFDALGAK + Oxidation (M)
3440 345.4384 1377.7245 1377.7252 -0.50 0 6 0.97 +1Score > 18 indicates identity KPDHQLAALQEK + Deamidated (NQ)
2703 822.4569 821.4496 821.4508 -1.38 1 6 0.55 +1Score > 16 indicates identity QRLSYR
2880 491.2019 980.3892 980.4055 -16.6 0 6 0.28 +1Score > 13 indicates identity MEMTNAQR + Deamidated (NQ)
3163 610.3038 1218.5930 1218.5880 4.12 1 6 1.3 +1Score > 19 indicates identity WEQIKTEER + Deamidated (NQ)
4458 1112.2657 3333.7753 3333.8169 -12.5 0 6 0.53 +1Score > 15 indicates identity LIPQMNYLMVVVALFFLNAVIFLFMLMK + Oxidation (M)
4374 658.3153 2629.2321 2629.2368 -1.80 1 6 1.2 +1Score > 19 indicates identity TTEQSWQQQTRTSQAAPVQAQPR + 3 Deamidated (NQ)
4032 659.6848 1976.0326 1976.0075 12.7 1 5 1.3 +1Score > 19 indicates identity EPRANTQAAEHILQEIR + Deamidated (NQ)
2604 700.3660 699.3587 699.3664 -10.9 0 5 0.43 +1Score > 14 indicates identity SLNPNR
2883 328.1480 981.4222 981.4039 18.6 0 5 0.41 +1Score > 14 indicates identity NPYEESSR + Deamidated (NQ)
3371 451.2219 1350.6439 1350.6602 -12.1 0 5 0.95 +1Score > 18 indicates identity NNVLSGLFCGLR + 2 Deamidated (NQ)
3520 474.5963 1420.7671 1420.7674 -0.23 1 5 0.62 +1Score > 16 indicates identity YQQLAKTNLVSR + Deamidated (NQ)
3537 476.5819 1426.7239 1426.7092 10.3 0 5 1.3 +1Score > 19 indicates identity LGIPDEALHNYGK + Deamidated (NQ)
2764 452.7305 903.4464 903.4297 18.5 1 5 1.5 +1Score > 19 indicates identity DNQQIKR + 3 Deamidated (NQ)
3460 694.8677 1387.7208 1387.7030 12.8 1 5 1.3 +1Score > 19 indicates identity RLMTASWPGNVR + Deamidated (NQ)
4071 1005.0213 2008.0280 2008.0126 7.68 1 5 1.2 +1Score > 19 indicates identity HRIQVVFQDPNSSLNPR + 2 Deamidated (NQ)
2859 478.7428 955.4710 955.4763 -5.52 0 5 0.43 +1Score > 14 indicates identity IASSFFQR + Deamidated (NQ)
3851 365.9889 1824.9081 1824.9152 -3.88 1 5 1.4 +1Score > 19 indicates identity SVNALMTATYQEIGRR + Oxidation (M)
4131 410.4030 2046.9786 2046.9761 1.22 1 5 1.3 +1Score > 19 indicates identity ENIMNNPVIGVVMCRNR + 2 Oxidation (M)
3480 697.8772 1393.7398 1393.7565 -12.0 1 5 0.8 +1Score > 16 indicates identity GAVPDAVADPNLKK
3416 341.9447 1363.7497 1363.7473 1.76 1 5 0.42 +1Score > 14 indicates identity FRHGIAIAGTHGK
3008 523.2681 1044.5216 1044.5200 1.62 1 5 1.3 +1Score > 19 indicates identity QDEQEIKR
3482 465.9085 1394.7037 1394.6929 7.70 0 5 1.1 +1Score > 18 indicates identity DVVTSYAQAVDVK + Deamidated (NQ)
3546 476.5838 1426.7296 1426.7092 14.3 0 5 1.6 +1Score > 19 indicates identity LGIPDEALHNYGK + Deamidated (NQ)
3683 564.3036 1689.8890 1689.8897 -0.45 1 5 1.3 +1Score > 19 indicates identity ADTTIISSGSRQVVEK
3968 645.3041 1932.8905 1932.9152 -12.8 1 5 1.1 +1Score > 18 indicates identity MSTPDNRSVNFFSLFR + Oxidation (M)
3170 409.8674 1226.5804 1226.5640 13.4 1 5 1 +1Score > 17 indicates identity RTTGNNNGGVHV + 2 Deamidated (NQ)
3143 601.3092 1200.6038 1200.6172 -11.1 1 5 1.6 +1Score > 19 indicates identity EAKIMDPQIR + Deamidated (NQ)
3536 476.5818 1426.7236 1426.7052 12.9 0 5 1.5 +1Score > 19 indicates identity LQQIEPNAGTEAR + Deamidated (NQ)
4047 663.3436 1987.0090 1986.9799 14.6 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology		 HYPLEADNIFAAIAATNR + Deamidated (NQ)
3497 351.9293 1403.6881 1403.6681 14.3 0 5 1.5 +1Score > 19 indicates identity ELAYNAPGNQGLR + 2 Deamidated (NQ)
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