MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1296 365.1595 364.1522
1297 365.1596 364.1523
1298 365.1596 364.1523
1299 365.1596 364.1523
1300 365.1599 364.1526
1301 365.1600 364.1527
1302 365.1601 364.1528
1303 365.1604 364.1531
1304 365.1944 364.1871
1305 365.1948 364.1875
1306 365.1949 364.1876
1307 365.1951 364.1878
1308 365.1952 364.1879
1309 365.1953 364.1880
1310 365.1954 364.1881
1311 365.1956 364.1883
1312 365.1958 364.1885
1313 365.1959 364.1886
1314 365.1959 364.1886
1315 365.1960 364.1887
1316 365.1960 364.1887
1317 365.1960 364.1887
1318 365.1962 364.1889
1319 365.1962 364.1889
1320 365.1962 364.1889
1321 365.1964 364.1891
1322 365.1969 364.1896
1323 365.2154 364.2081
1324 365.2155 364.2082
1325 365.2159 364.2086
1326 365.2159 364.2086
1327 367.0478 366.0405
1328 367.0727 366.0654
1329 367.0739 366.0666
1330 367.1368 366.1295
1331 367.1374 366.1301
1332 367.1374 366.1301
1333 367.1378 366.1305
1334 367.1378 366.1305
1335 367.1378 366.1305
1336 367.1380 366.1307
1337 367.1384 366.1311
1338 367.1385 366.1312
1339 367.1385 366.1312
1340 367.1388 366.1315
1341 367.1389 366.1316
1342 367.1738 366.1665
1343 367.1740 366.1667
1344 367.1742 366.1669
1345 367.1743 366.1670
1346 367.1743 366.1670
1347 367.1743 366.1670
1348 367.1743 366.1670
1349 367.1744 366.1671
1350 367.1745 366.1672
1351 367.1745 366.1672
1352 367.1748 366.1675
1353 367.1750 366.1677
1354 367.1750 366.1677
1355 367.1750 366.1677
1356 367.1751 366.1678
1357 367.1752 366.1679
1358 367.1753 366.1680
1359 367.1754 366.1681
1360 367.1756 366.1683
1361 367.1757 366.1684
1362 367.1758 366.1685
1363 367.1921 366.1848
1364 367.2095 366.2022
1365 367.2115 366.2042
1366 367.2120 366.2047
1367 367.2121 366.2048
1368 367.2124 366.2051
1369 367.2322 366.2249
1370 367.2325 366.2252
1371 367.2325 366.2252
1372 367.2328 366.2255
1373 367.2351 366.2278
1374 367.2466 366.2393
1375 368.2310 367.2237
1376 369.0881 368.0808
1377 369.0888 368.0815
1378 369.1183 368.1110
1379 369.1230 368.1157
1380 369.1333 368.1260
1381 369.1410 368.1337
1382 369.1527 368.1454
1383 369.1531 368.1458
1384 369.1534 368.1461
1385 369.1536 368.1463
1386 369.1539 368.1466
1387 369.1542 368.1469
1388 369.1542 368.1469
1389 369.1545 368.1472
1390 369.1546 368.1473
1391 369.1896 368.1823
1392 369.1898 368.1825
1393 369.1904 368.1831
1394 369.1907 368.1834
1395 369.1907 368.1834
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