MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1196 357.1899 356.1826
1197 357.1903 356.1830
1198 357.2266 356.2193
1199 359.0214 358.0141
1200 359.0662 358.0589
1201 359.1038 358.0965
1202 359.1113 358.1040
1203 359.1124 358.1051
1204 359.1138 358.1065
1205 359.1476 358.1403
1206 359.1490 358.1417
1207 359.1704 358.1631
1208 359.2050 358.1977
1209 359.2056 358.1983
1210 359.2411 358.2338
1211 361.0899 360.0826
1212 361.1271 360.1198
1213 361.1274 360.1201
1214 361.1281 360.1208
1215 361.1632 360.1559
1216 361.1635 360.1562
1217 361.1636 360.1563
1218 361.1636 360.1563
1219 361.1645 360.1572
1220 361.1645 360.1572
1221 361.1646 360.1573
1222 361.1646 360.1573
1223 361.1646 360.1573
1224 361.1647 360.1574
1225 361.1647 360.1574
1226 361.1647 360.1574
1227 361.1650 360.1577
1228 361.1650 360.1577
1229 361.2005 360.1932
1230 361.2009 360.1936
1231 361.2010 360.1937
1232 361.2011 360.1938
1233 361.2014 360.1941
1234 361.2455 360.2382
1235 361.2455 360.2382
1236 361.2462 360.2389
1237 361.2469 360.2396
1238 363.0534 362.0461
1239 363.1045 362.0972
1240 363.1051 362.0978
1241 363.1425 362.1352
1242 363.1426 362.1353
1243 363.1426 362.1353
1244 363.1428 362.1355
1245 363.1429 362.1356
1246 363.1430 362.1357
1247 363.1431 362.1358
1248 363.1433 362.1360
1249 363.1437 362.1364
1250 363.1438 362.1365
1251 363.1438 362.1365
1252 363.1439 362.1366
1253 363.1441 362.1368
1254 363.1441 362.1368
1255 363.1778 362.1705
1256 363.1784 362.1711
1257 363.1787 362.1714
1258 363.1790 362.1717
1259 363.1790 362.1717
1260 363.1792 362.1719
1261 363.1794 362.1721
1262 363.1800 362.1727
1263 363.1800 362.1727
1264 363.1801 362.1728
1265 363.1801 362.1728
1266 363.1803 362.1730
1267 363.1803 362.1730
1268 363.1803 362.1730
1269 363.1804 362.1731
1270 363.1805 362.1732
1271 363.1805 362.1732
1272 363.1805 362.1732
1273 363.1806 362.1733
1274 363.1807 362.1734
1275 363.1808 362.1735
1276 363.2173 362.2100
1277 364.2346 363.2273
1278 365.0314 364.0241
1279 365.0328 364.0255
1280 365.0840 364.0767
1281 365.1212 364.1139
1282 365.1221 364.1148
1283 365.1222 364.1149
1284 365.1223 364.1150
1285 365.1231 364.1158
1286 365.1580 364.1507
1287 365.1581 364.1508
1288 365.1581 364.1508
1289 365.1582 364.1509
1290 365.1584 364.1511
1291 365.1586 364.1513
1292 365.1586 364.1513
1293 365.1587 364.1514
1294 365.1588 364.1515
1295 365.1594 364.1521
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