MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1096 351.1805 350.1732
1097 351.1807 350.1734
1098 351.1989 350.1916
1099 351.1992 350.1919
1100 351.2153 350.2080
1101 351.2153 350.2080
1102 351.2158 350.2085
1103 351.2159 350.2086
1104 351.2161 350.2088
1105 351.2165 350.2092
1106 351.2167 350.2094
1107 351.2167 350.2094
1108 351.2168 350.2095
1109 351.2172 350.2099
1110 351.2363 350.2290
1111 351.2363 350.2290
1112 351.2367 350.2294
1113 351.2369 350.2296
1114 351.2371 350.2298
1115 353.0936 352.0863
1116 353.0938 352.0865
1117 353.1097 352.1024
1118 353.1208 352.1135
1119 353.1212 352.1139
1120 353.1213 352.1140
1121 353.1214 352.1141
1122 353.1217 352.1144
1123 353.1219 352.1146
1124 353.1222 352.1149
1125 353.1223 352.1150
1126 353.1228 352.1155
1127 353.1578 352.1505
1128 353.1581 352.1508
1129 353.1582 352.1509
1130 353.1582 352.1509
1131 353.1583 352.1510
1132 353.1584 352.1511
1133 353.1584 352.1511
1134 353.1585 352.1512
1135 353.1586 352.1513
1136 353.1586 352.1513
1137 353.1592 352.1519
1138 353.1594 352.1521
1139 353.1595 352.1522
1140 353.1598 352.1525
1141 353.1598 352.1525
1142 353.1599 352.1526
1143 353.1947 352.1874
1144 353.1955 352.1882
1145 353.2323 352.2250
1146 353.2529 352.2456
1147 353.2530 352.2457
1148 354.2360 353.2287
1149 355.0113 354.0040
1150 355.0717 354.0644
1151 355.0718 354.0645
1152 355.1069 354.0996
1153 355.1080 354.1007
1154 355.1082 354.1009
1155 355.1096 354.1023
1156 355.1360 354.1287
1157 355.1369 354.1296
1158 355.1374 354.1301
1159 355.1378 354.1305
1160 355.1381 354.1308
1161 355.1382 354.1309
1162 355.1383 354.1310
1163 355.1384 354.1311
1164 355.1387 354.1314
1165 355.1389 354.1316
1166 355.1733 354.1660
1167 355.1736 354.1663
1168 355.1742 354.1669
1169 355.1743 354.1670
1170 355.1748 354.1675
1171 355.1751 354.1678
1172 355.1752 354.1679
1173 355.1933 354.1860
1174 355.1935 354.1862
1175 355.1942 354.1869
1176 355.2691 354.2618
1177 356.1790 355.1717
1178 356.2820 355.2747
1179 356.2820 355.2747
1180 356.2823 355.2750
1181 356.2827 355.2754
1182 357.0857 356.0784
1183 357.0865 356.0792
1184 357.0871 356.0798
1185 357.0873 356.0800
1186 357.0875 356.0802
1187 357.0876 356.0803
1188 357.1257 356.1184
1189 357.1258 356.1185
1190 357.1526 356.1453
1191 357.1534 356.1461
1192 357.1538 356.1465
1193 357.1541 356.1468
1194 357.1543 356.1470
1195 357.1546 356.1473
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