MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
996 347.1853 346.1780
997 347.1853 346.1780
998 347.1854 346.1781
999 347.1855 346.1782
1000 347.1860 346.1787
1001 347.2300 346.2227
1002 348.2032 347.1959
1003 349.0315 348.0242
1004 349.1255 348.1182
1005 349.1263 348.1190
1006 349.1272 348.1199
1007 349.1272 348.1199
1008 349.1272 348.1199
1009 349.1273 348.1200
1010 349.1274 348.1201
1011 349.1276 348.1203
1012 349.1278 348.1205
1013 349.1279 348.1206
1014 349.1279 348.1206
1015 349.1631 348.1558
1016 349.1632 348.1559
1017 349.1633 348.1560
1018 349.1633 348.1560
1019 349.1634 348.1561
1020 349.1635 348.1562
1021 349.1635 348.1562
1022 349.1636 348.1563
1023 349.1636 348.1563
1024 349.1637 348.1564
1025 349.1637 348.1564
1026 349.1644 348.1571
1027 349.1645 348.1572
1028 349.1645 348.1572
1029 349.1645 348.1572
1030 349.1646 348.1573
1031 349.1646 348.1573
1032 349.1646 348.1573
1033 349.1647 348.1574
1034 349.1647 348.1574
1035 349.1647 348.1574
1036 349.1652 348.1579
1037 349.1851 348.1778
1038 349.1994 348.1921
1039 349.1998 348.1925
1040 349.2000 348.1927
1041 349.2002 348.1929
1042 349.2003 348.1930
1043 349.2003 348.1930
1044 349.2006 348.1933
1045 349.2007 348.1934
1046 349.2009 348.1936
1047 349.2009 348.1936
1048 349.2010 348.1937
1049 349.2010 348.1937
1050 349.2010 348.1937
1051 349.2011 348.1938
1052 349.2012 348.1939
1053 349.2016 348.1943
1054 349.2209 348.2136
1055 349.2211 348.2138
1056 349.2214 348.2141
1057 349.2218 348.2145
1058 349.2224 348.2151
1059 351.0501 350.0428
1060 351.0777 350.0704
1061 351.1422 350.1349
1062 351.1423 350.1350
1063 351.1423 350.1350
1064 351.1424 350.1351
1065 351.1425 350.1352
1066 351.1426 350.1353
1067 351.1428 350.1355
1068 351.1428 350.1355
1069 351.1432 350.1359
1070 351.1432 350.1359
1071 351.1435 350.1362
1072 351.1435 350.1362
1073 351.1436 350.1363
1074 351.1436 350.1363
1075 351.1436 350.1363
1076 351.1789 350.1716
1077 351.1790 350.1717
1078 351.1790 350.1717
1079 351.1790 350.1717
1080 351.1792 350.1719
1081 351.1792 350.1719
1082 351.1793 350.1720
1083 351.1793 350.1720
1084 351.1795 350.1722
1085 351.1797 350.1724
1086 351.1797 350.1724
1087 351.1797 350.1724
1088 351.1799 350.1726
1089 351.1800 350.1727
1090 351.1801 350.1728
1091 351.1802 350.1729
1092 351.1803 350.1730
1093 351.1803 350.1730
1094 351.1804 350.1731
1095 351.1804 350.1731
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