MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
896 339.1991 338.1918
897 339.2737 338.2664
898 341.0556 340.0483
899 341.0913 340.0840
900 341.0919 340.0846
901 341.0921 340.0848
902 341.0933 340.0860
903 341.1099 340.1026
904 341.1100 340.1027
905 341.1208 340.1135
906 341.1213 340.1140
907 341.1219 340.1146
908 341.1219 340.1146
909 341.1221 340.1148
910 341.1224 340.1151
911 341.1228 340.1155
912 341.1231 340.1158
913 341.1577 340.1504
914 341.1580 340.1507
915 341.1581 340.1508
916 341.1584 340.1511
917 341.1584 340.1511
918 341.1584 340.1511
919 341.1585 340.1512
920 341.1586 340.1513
921 341.1586 340.1513
922 341.1590 340.1517
923 341.1591 340.1518
924 341.1592 340.1519
925 341.1592 340.1519
926 341.1593 340.1520
927 341.1952 340.1879
928 341.1959 340.1886
929 341.1960 340.1887
930 341.1961 340.1888
931 341.1961 340.1888
932 341.1963 340.1890
933 341.1964 340.1891
934 341.1966 340.1893
935 341.2167 340.2094
936 342.1928 341.1855
937 343.0705 342.0632
938 343.0711 342.0638
939 343.0716 342.0643
940 343.1091 342.1018
941 343.1741 342.1668
942 343.1742 342.1669
943 343.1745 342.1672
944 343.1748 342.1675
945 343.1933 342.1860
946 344.1990 343.1917
947 344.2081 343.2008
948 344.2088 343.2015
949 344.2089 343.2016
950 345.0504 344.0431
951 345.0857 344.0784
952 345.0877 344.0804
953 345.1320 344.1247
954 345.1322 344.1249
955 345.1328 344.1255
956 345.1330 344.1257
957 345.1695 344.1622
958 345.1695 344.1622
959 345.1699 344.1626
960 345.1702 344.1629
961 345.4384 344.4311
962 345.6893 344.6820
963 346.2237 345.2164
964 346.2238 345.2165
965 346.2246 345.2173
966 346.2248 345.2175
967 347.1111 346.1038
968 347.1119 346.1046
969 347.1475 346.1402
970 347.1475 346.1402
971 347.1476 346.1403
972 347.1477 346.1404
973 347.1478 346.1405
974 347.1479 346.1406
975 347.1480 346.1407
976 347.1486 346.1413
977 347.1487 346.1414
978 347.1487 346.1414
979 347.1488 346.1415
980 347.1489 346.1416
981 347.1489 346.1416
982 347.1492 346.1419
983 347.1838 346.1765
984 347.1839 346.1766
985 347.1840 346.1767
986 347.1842 346.1769
987 347.1843 346.1770
988 347.1845 346.1772
989 347.1850 346.1777
990 347.1851 346.1778
991 347.1851 346.1778
992 347.1852 346.1779
993 347.1852 346.1779
994 347.1852 346.1779
995 347.1852 346.1779
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