MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
796 337.0341 336.0268
797 337.0889 336.0816
798 337.0984 336.0911
799 337.1079 336.1006
800 337.1252 336.1179
801 337.1265 336.1192
802 337.1266 336.1193
803 337.1267 336.1194
804 337.1270 336.1197
805 337.1270 336.1197
806 337.1271 336.1198
807 337.1272 336.1199
808 337.1275 336.1202
809 337.1276 336.1203
810 337.1279 336.1206
811 337.1281 336.1208
812 337.1284 336.1211
813 337.1628 336.1555
814 337.1631 336.1558
815 337.1631 336.1558
816 337.1633 336.1560
817 337.1633 336.1560
818 337.1634 336.1561
819 337.1634 336.1561
820 337.1635 336.1562
821 337.1636 336.1563
822 337.1636 336.1563
823 337.1637 336.1564
824 337.1637 336.1564
825 337.1638 336.1565
826 337.1640 336.1567
827 337.1642 336.1569
828 337.1643 336.1570
829 337.1643 336.1570
830 337.1643 336.1570
831 337.1643 336.1570
832 337.1644 336.1571
833 337.1646 336.1573
834 337.1647 336.1574
835 337.1647 336.1574
836 337.1814 336.1741
837 337.1829 336.1756
838 337.1833 336.1760
839 337.1835 336.1762
840 337.2012 336.1939
841 337.2013 336.1940
842 337.2378 336.2305
843 337.2571 336.2498
844 338.1512 337.1439
845 338.1839 337.1766
846 338.2409 337.2336
847 338.6765 337.6692
848 339.0159 338.0086
849 339.0764 338.0691
850 339.0771 338.0698
851 339.0772 338.0699
852 339.0939 338.0866
853 339.1045 338.0972
854 339.1046 338.0973
855 339.1052 338.0979
856 339.1053 338.0980
857 339.1060 338.0987
858 339.1067 338.0994
859 339.1072 338.0999
860 339.1131 338.1058
861 339.1133 338.1060
862 339.1135 338.1062
863 339.1217 338.1144
864 339.1226 338.1153
865 339.1419 338.1346
866 339.1420 338.1347
867 339.1421 338.1348
868 339.1424 338.1351
869 339.1424 338.1351
870 339.1425 338.1352
871 339.1425 338.1352
872 339.1427 338.1354
873 339.1427 338.1354
874 339.1428 338.1355
875 339.1428 338.1355
876 339.1428 338.1355
877 339.1429 338.1356
878 339.1429 338.1356
879 339.1429 338.1356
880 339.1431 338.1358
881 339.1432 338.1359
882 339.1435 338.1362
883 339.1437 338.1364
884 339.1438 338.1365
885 339.1439 338.1366
886 339.1680 338.1607
887 339.1791 338.1718
888 339.1795 338.1722
889 339.1795 338.1722
890 339.1796 338.1723
891 339.1798 338.1725
892 339.1800 338.1727
893 339.1802 338.1729
894 339.1989 338.1916
895 339.1989 338.1916
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