MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
696 333.1687 332.1614
697 333.1687 332.1614
698 333.1687 332.1614
699 333.1688 332.1615
700 333.1692 332.1619
701 333.1693 332.1620
702 333.1693 332.1620
703 333.1695 332.1622
704 333.1696 332.1623
705 333.1698 332.1625
706 333.1699 332.1626
707 333.1700 332.1627
708 333.1702 332.1629
709 333.1703 332.1630
710 333.1916 332.1843
711 333.2045 332.1972
712 333.2047 332.1974
713 333.2050 332.1977
714 333.2054 332.1981
715 333.2056 332.1983
716 333.2056 332.1983
717 333.2057 332.1984
718 333.2057 332.1984
719 333.2057 332.1984
720 333.2058 332.1985
721 333.2058 332.1985
722 333.2060 332.1987
723 333.2060 332.1987
724 333.2064 332.1991
725 333.2268 332.2195
726 334.2243 333.2170
727 334.2244 333.2171
728 335.0042 333.9969
729 335.0737 334.0664
730 335.0919 334.0846
731 335.1096 334.1023
732 335.1099 334.1026
733 335.1100 334.1027
734 335.1102 334.1029
735 335.1106 334.1033
736 335.1109 334.1036
737 335.1115 334.1042
738 335.1116 334.1043
739 335.1118 334.1045
740 335.1121 334.1048
741 335.1472 334.1399
742 335.1475 334.1402
743 335.1475 334.1402
744 335.1476 334.1403
745 335.1477 334.1404
746 335.1478 334.1405
747 335.1478 334.1405
748 335.1479 334.1406
749 335.1479 334.1406
750 335.1480 334.1407
751 335.1482 334.1409
752 335.1483 334.1410
753 335.1484 334.1411
754 335.1485 334.1412
755 335.1486 334.1413
756 335.1486 334.1413
757 335.1487 334.1414
758 335.1489 334.1416
759 335.1490 334.1417
760 335.1841 334.1768
761 335.1843 334.1770
762 335.1844 334.1771
763 335.1845 334.1772
764 335.1845 334.1772
765 335.1847 334.1774
766 335.1849 334.1776
767 335.1852 334.1779
768 335.1852 334.1779
769 335.1852 334.1779
770 335.1852 334.1779
771 335.1853 334.1780
772 335.1853 334.1780
773 335.1854 334.1781
774 335.1856 334.1783
775 335.1858 334.1785
776 335.2203 334.2130
777 335.2203 334.2130
778 335.2207 334.2134
779 335.2209 334.2136
780 335.2211 334.2138
781 335.2213 334.2140
782 335.2214 334.2141
783 335.2215 334.2142
784 335.2215 334.2142
785 335.2215 334.2142
786 335.2215 334.2142
787 335.2217 334.2144
788 335.2221 334.2148
789 335.2426 334.2353
790 335.9292 334.9219
791 335.9294 334.9221
792 335.9294 334.9221
793 336.2249 335.2176
794 336.8284 335.8211
795 337.0339 336.0266
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