MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
596 327.1066 326.0993
597 327.1067 326.0994
598 327.1136 326.1063
599 327.1228 326.1155
600 327.1235 326.1162
601 327.1422 326.1349
602 327.1423 326.1350
603 327.1425 326.1352
604 327.1425 326.1352
605 327.1425 326.1352
606 327.1427 326.1354
607 327.1429 326.1356
608 327.1430 326.1357
609 327.1430 326.1357
610 327.1431 326.1358
611 327.1432 326.1359
612 327.1432 326.1359
613 327.1433 326.1360
614 327.1434 326.1361
615 327.1434 326.1361
616 327.1435 326.1362
617 327.1436 326.1363
618 327.1786 326.1713
619 327.1787 326.1714
620 327.1789 326.1716
621 327.1793 326.1720
622 327.1794 326.1721
623 327.1798 326.1725
624 327.1799 326.1726
625 327.1800 326.1727
626 327.2003 326.1930
627 327.2177 326.2104
628 328.1655 327.1582
629 328.2139 327.2066
630 328.2139 327.2066
631 328.6625 327.6552
632 328.9870 327.9797
633 329.0555 328.0482
634 329.0556 328.0483
635 329.0556 328.0483
636 329.0911 328.0838
637 329.0914 328.0841
638 329.0930 328.0857
639 329.1223 328.1150
640 329.1580 328.1507
641 329.1581 328.1508
642 329.1582 328.1509
643 329.1583 328.1510
644 329.1585 328.1512
645 329.1586 328.1513
646 329.1587 328.1514
647 329.1588 328.1515
648 329.1591 328.1518
649 329.1591 328.1518
650 329.1591 328.1518
651 329.1592 328.1519
652 329.1945 328.1872
653 329.1956 328.1883
654 330.0416 329.0343
655 331.0712 330.0639
656 331.0715 330.0642
657 331.1162 330.1089
658 331.1164 330.1091
659 331.1534 330.1461
660 331.1535 330.1462
661 331.1537 330.1464
662 331.1537 330.1464
663 331.1538 330.1465
664 331.1539 330.1466
665 331.1540 330.1467
666 331.1769 330.1696
667 331.1898 330.1825
668 331.1901 330.1828
669 331.1902 330.1829
670 331.1904 330.1831
671 331.1906 330.1833
672 332.0575 331.0502
673 332.2087 331.2014
674 332.2088 331.2015
675 333.0564 332.0491
676 333.0571 332.0498
677 333.0573 332.0500
678 333.0875 332.0802
679 333.0939 332.0866
680 333.0943 332.0870
681 333.0949 332.0876
682 333.1319 332.1246
683 333.1320 332.1247
684 333.1320 332.1247
685 333.1320 332.1247
686 333.1321 332.1248
687 333.1322 332.1249
688 333.1323 332.1250
689 333.1328 332.1255
690 333.1329 332.1256
691 333.1329 332.1256
692 333.1329 332.1256
693 333.1552 332.1479
694 333.1685 332.1612
695 333.1685 332.1612
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