MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
496 323.1111 322.1038
497 323.1111 322.1038
498 323.1115 322.1042
499 323.1115 322.1042
500 323.1115 322.1042
501 323.1115 322.1042
502 323.1116 322.1043
503 323.1118 322.1045
504 323.1472 322.1399
505 323.1472 322.1399
506 323.1474 322.1401
507 323.1475 322.1402
508 323.1475 322.1402
509 323.1476 322.1403
510 323.1476 322.1403
511 323.1477 322.1404
512 323.1479 322.1406
513 323.1479 322.1406
514 323.1479 322.1406
515 323.1480 322.1407
516 323.1482 322.1409
517 323.1483 322.1410
518 323.1484 322.1411
519 323.1486 322.1413
520 323.1486 322.1413
521 323.1486 322.1413
522 323.1487 322.1414
523 323.1487 322.1414
524 323.1490 322.1417
525 323.1657 322.1584
526 323.1662 322.1589
527 323.1664 322.1591
528 323.1666 322.1593
529 323.1839 322.1766
530 323.1841 322.1768
531 323.1841 322.1768
532 323.1843 322.1770
533 323.1844 322.1771
534 323.1845 322.1772
535 323.1845 322.1772
536 323.1846 322.1773
537 323.1850 322.1777
538 323.1851 322.1778
539 323.1852 322.1779
540 323.1852 322.1779
541 323.1852 322.1779
542 323.1853 322.1780
543 323.1853 322.1780
544 323.1854 322.1781
545 323.1855 322.1782
546 323.2048 322.1975
547 325.0612 324.0539
548 325.0905 324.0832
549 325.0988 324.0915
550 325.0999 324.0926
551 325.1068 324.0995
552 325.1072 324.0999
553 325.1265 324.1192
554 325.1266 324.1193
555 325.1266 324.1193
556 325.1267 324.1194
557 325.1270 324.1197
558 325.1271 324.1198
559 325.1272 324.1199
560 325.1273 324.1200
561 325.1274 324.1201
562 325.1275 324.1202
563 325.1275 324.1202
564 325.1275 324.1202
565 325.1276 324.1203
566 325.1277 324.1204
567 325.1279 324.1206
568 325.1632 324.1559
569 325.1633 324.1560
570 325.1635 324.1562
571 325.1636 324.1563
572 325.1636 324.1563
573 325.1637 324.1564
574 325.1637 324.1564
575 325.1641 324.1568
576 325.1642 324.1569
577 325.1642 324.1569
578 325.1643 324.1570
579 325.1644 324.1571
580 325.1821 324.1748
581 325.1822 324.1749
582 325.1824 324.1751
583 325.1829 324.1756
584 325.2013 324.1940
585 325.2018 324.1945
586 326.1486 325.1413
587 327.0756 326.0683
588 327.0757 326.0684
589 327.0758 326.0685
590 327.0763 326.0690
591 327.0765 326.0692
592 327.0767 326.0694
593 327.1050 326.0977
594 327.1052 326.0979
595 327.1060 326.0987
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