MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4435)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
396 319.1169 318.1096
397 319.1169 318.1096
398 319.1171 318.1098
399 319.1524 318.1451
400 319.1529 318.1456
401 319.1529 318.1456
402 319.1531 318.1458
403 319.1531 318.1458
404 319.1534 318.1461
405 319.1534 318.1461
406 319.1535 318.1462
407 319.1535 318.1462
408 319.1535 318.1462
409 319.1536 318.1463
410 319.1536 318.1463
411 319.1537 318.1464
412 319.1537 318.1464
413 319.1538 318.1465
414 319.1538 318.1465
415 319.1893 318.1820
416 319.1898 318.1825
417 319.1901 318.1828
418 319.1901 318.1828
419 319.1902 318.1829
420 319.1903 318.1830
421 319.1903 318.1830
422 319.1906 318.1833
423 319.2264 318.2191
424 319.2266 318.2193
425 319.2267 318.2194
426 319.2270 318.2197
427 319.9946 318.9873
428 319.9953 318.9880
429 321.0649 320.0576
430 321.0672 320.0599
431 321.0934 320.0861
432 321.0938 320.0865
433 321.0941 320.0868
434 321.0944 320.0871
435 321.0945 320.0872
436 321.0950 320.0877
437 321.0952 320.0879
438 321.0956 320.0883
439 321.0960 320.0887
440 321.1301 320.1228
441 321.1306 320.1233
442 321.1310 320.1237
443 321.1316 320.1243
444 321.1317 320.1244
445 321.1317 320.1244
446 321.1318 320.1245
447 321.1318 320.1245
448 321.1319 320.1246
449 321.1320 320.1247
450 321.1320 320.1247
451 321.1320 320.1247
452 321.1320 320.1247
453 321.1321 320.1248
454 321.1327 320.1254
455 321.1328 320.1255
456 321.1328 320.1255
457 321.1328 320.1255
458 321.1328 320.1255
459 321.1329 320.1256
460 321.1680 320.1607
461 321.1682 320.1609
462 321.1684 320.1611
463 321.1684 320.1611
464 321.1685 320.1612
465 321.1687 320.1614
466 321.1687 320.1614
467 321.1688 320.1615
468 321.1688 320.1615
469 321.1690 320.1617
470 321.1692 320.1619
471 321.1693 320.1620
472 321.1693 320.1620
473 321.1693 320.1620
474 321.1694 320.1621
475 321.1694 320.1621
476 321.1694 320.1621
477 321.1694 320.1621
478 321.1695 320.1622
479 321.1696 320.1623
480 321.2059 320.1986
481 321.2060 320.1987
482 321.2060 320.1987
483 321.2062 320.1989
484 321.2423 320.2350
485 321.2424 320.2351
486 321.2426 320.2353
487 322.1562 321.1489
488 322.1565 321.1492
489 322.1565 321.1492
490 323.0186 322.0113
491 323.0485 322.0412
492 323.1105 322.1032
493 323.1105 322.1032
494 323.1107 322.1034
495 323.1108 322.1035
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