MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1249__1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues) |
| Timestamp | : | 23 Jun 2025 at 17:07:31 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,592 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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31| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NFRA_ECOLI | 18 | Bacteriophage N4 adsorption protein A OS=Escherichia coli (strain K12) GN=nfrA PE=4 SV=1 |
32| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADEC_ECOLI | 18 | Adenine deaminase OS=Escherichia coli (strain K12) GN=ade PE=1 SV=2 |
33| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q1XAB0_CHOFU | 18 | Putative farnesyl diphosphate synthase OS=Choristoneura fumiferana PE=2 SV=1 |
34| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PGAC_ECOLI | 16 | Poly-beta-1,6-N-acetyl-D-glucosamine synthase OS=Escherichia coli (strain K12) GN=pgaC PE=1 SV=1 |
35| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | A4U8R3_9BACT | 16 | SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 35.1 | A4U8R3_9BACT |
16 | 362145 | 6 (1) | 5 (1) | 0.01 | |
| SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1 | |||||||
| 1 sameset of A4U8R3_9BACT | |||||||
| Q70HZ8_9ACTN |
16 | 0 | 2 (1) | 1 (1) | 0.03 | ||
| description | |||||||
6 peptide matches (5 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2424 |
1 |
541.3469 | 540.3396 | 540.3384 | 2.30 | 0 | 16 | 0.025 | 1Score > 13 indicates identity |
U | R.GVIPR.H |
| 2469 |
590.2905 | 589.2832 | 589.2820 | 2.13 | 0 | 4 | 1.2 | 8Score > 17 indicates identity |
U | R.ATENR.D | |
| 2855 |
955.5176 | 954.5103 | 954.5247 | -15.0 | 1 | 0 | 2.5 | 1Score > 17 indicates identity |
U | R.RLEPGEVR.A | |
| 4073 |
1005.0219 | 2008.0292 | 2008.0564 | -13.5 | 0 | 2 | 2.7 | 5Score > 19 indicates identity |
U | R.GGLIGALPGDGAMAAVFAPAPR.V | |
| 4257 |
819.7629 | 2456.2669 | 2456.2879 | -8.57 | 1 | 3 | 2.2 | 1Score > 19 indicates identity |
U | K.TSIGHLESAAGIAGLMKTVLAMNR.G + Oxidation (M) |
36| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PSUT_ECOLI | 15 | description |
37| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 14 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
38| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HLYE_ECOLI | 13 | Hemolysin E, chromosomal OS=Escherichia coli (strain K12) GN=hlyE PE=1 SV=4 |
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