MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–38 (out of 38)


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+31

Accession Score Description
1 NFRA_ECOLI 18 Bacteriophage N4 adsorption protein A OS=Escherichia coli (strain K12) GN=nfrA PE=4 SV=1

+32

Accession Score Description
1 ADEC_ECOLI 18 Adenine deaminase OS=Escherichia coli (strain K12) GN=ade PE=1 SV=2

-33

Accession Score Description
1 Q1XAB0_CHOFU 18 Putative farnesyl diphosphate synthase OS=Choristoneura fumiferana PE=2 SV=1
Score Mass Matches Sequences emPAI
33.1 Q1XAB0_CHOFU 18 51396 2 (1) 2 (1) 0.06
Putative farnesyl diphosphate synthase OS=Choristoneura fumiferana PE=2 SV=1

+2 peptide matches (2 non-duplicate, 0 duplicate)


+34

Accession Score Description
1 PGAC_ECOLI 16 Poly-beta-1,6-N-acetyl-D-glucosamine synthase OS=Escherichia coli (strain K12) GN=pgaC PE=1 SV=1

+35

Accession Score Description
1 A4U8R3_9BACT 16 SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1

+36

Accession Score Description
1 PSUT_ECOLI 15 description

+37

Accession Score Description
1 CH60_ECOLI 14 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2

+38

Accession Score Description
1 HLYE_ECOLI 13 Hemolysin E, chromosomal OS=Escherichia coli (strain K12) GN=hlyE PE=1 SV=4
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