MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 38)

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+21

Accession Score Description
1 FABB_ECOLI 22 3-oxoacyl-[acyl-carrier-protein] synthase 1 OS=Escherichia coli (strain K12) GN=fabB PE=1 SV=1

+22

Accession Score Description
1 GSH1_ECOLI 22 Glutamate--cysteine ligase OS=Escherichia coli (strain K12) GN=gshA PE=1 SV=1

+23

Accession Score Description
1 NARQ_ECOLI 21 description

-24

Accession Score Description
1 YHII_ECOLI 20 Uncharacterized protein YhiI OS=Escherichia coli (strain K12) GN=yhiI PE=4 SV=1
Score Mass Matches Sequences emPAI
24.1 YHII_ECOLI 20 38805 3 (1) 3 (1) 0.09
Uncharacterized protein YhiI OS=Escherichia coli (strain K12) GN=yhiI PE=4 SV=1
1 sameset of YHII_ECOLI
FRLR_ECOLI 20 28088 2 (1) 1 (1) 0.12
HTH-type transcriptional regulator FrlR OS=Escherichia coli (strain K12) GN=frlR PE=4 SV=2

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2556   671.3510 670.3437 670.3511 -10.9 1 9 0.12 +1Score > 13 indicates identity U K.APRDGR.V
2679   387.7097 773.4048 773.3919 16.7 0 20 0.044 +1Score > 19 indicates identity U R.VLQEQR.L + 2 Deamidated (NQ)
2907   988.5101 987.5028 987.4985 4.39 0 4 2 +3Score > 19 indicates identity U R.AAQSLVNQR.Q + 2 Deamidated (NQ)

+25

Accession Score Description
1 FLIG_ECOLI 20 Flagellar motor switch protein FliG OS=Escherichia coli (strain K12) GN=fliG PE=1 SV=1

+26

Accession Score Description
1 MOAE_ECOLI 20 description

+27

Accession Score Description
1 RSMA_ECOLI 20 Ribosomal RNA small subunit methyltransferase A OS=Escherichia coli (strain K12) GN=rsmA PE=1 SV=1

+28

Accession Score Description
1 YBIP_ECOLI 20 description

+29

Accession Score Description
1 YNIA_ECOLI 19 Uncharacterized protein YniA OS=Escherichia coli (strain K12) GN=yniA PE=3 SV=1

+30

Accession Score Description
1 GNTR_ECOLI 18 HTH-type transcriptional regulator GntR OS=Escherichia coli (strain K12) GN=gntR PE=1 SV=1
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