MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1249__1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues) |
| Timestamp | : | 23 Jun 2025 at 17:07:31 GMT |
Not what you expected? Try 
the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,592 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SAPF_ECOLI | 29 | Peptide transport system ATP-binding protein SapF OS=Escherichia coli (strain K12) GN=sapF PE=1 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DAMX_ECOLI | 28 | Protein DamX OS=Escherichia coli (strain K12) GN=damX PE=1 SV=2 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YNJD_ECOLI | 27 | Uncharacterized ABC transporter ATP-binding protein YnjD OS=Escherichia coli (strain K12) GN=ynjD PE=3 SV=2 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ZUR_ECOLI | 26 | Zinc uptake regulation protein OS=Escherichia coli (strain K12) GN=zur PE=1 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | VIOA_CHRVO | 26 | Probable L-tryptophan oxidase VioA OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) GN=vioA PE=1 SV=2 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CMOB_ECOLI | 25 | tRNA (mo5U34)-methyltransferase OS=Escherichia coli (strain K12) GN=cmoB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 16.1 | CMOB_ECOLI |
25 | 37154 | 6 (1) | 2 (1) | 0.09 | |
| tRNA (mo5U34)-methyltransferase OS=Escherichia coli (strain K12) GN=cmoB PE=3 SV=1 | |||||||
| 2 samesets of CMOB_ECOLI | |||||||
| J9WMQ1_AT-TE-9ACTN |
25 | 275009 | 6 (1) | 2 (1) | 0.01 | ||
| Flv002-AT-TE-FluA OS=Actinomadura vulgaris subsp. lanata OX=1233072 GN=fluA PE=4 SV=1 | |||||||
| HTRE_ECOLI |
25 | 95783 | 5 (1) | 1 (1) | 0.03 | ||
| Outer membrane usher protein HtrE OS=Escherichia coli (strain K12) GN=htrE PE=1 SV=3 | |||||||
6 peptide matches (3 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2511 |
2 |
321.7190 | 641.4234 | 641.4224 | 1.60 | 0 | 13 | 0.066 | 1Score > 14 indicates identity |
U | R.AVLIAR.K |
| 2516 |
1 |
642.4322 | 641.4249 | 641.4224 | 3.90 | 0 | 25 | 0.0037 | 1Score > 14 indicates identity |
U | R.AVLIAR.K |
| 2695 |
408.7214 | 815.4282 | 815.4137 | 17.8 | 0 | 16 | 0.14 | 4Score > 20 indicates identity |
U | K.LLGNDQR.A + Deamidated (NQ) |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_ECOLI | 25 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TOP3_ECOLI | 25 | DNA topoisomerase 3 OS=Escherichia coli (strain K12) GN=topB PE=1 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RTCR_ECOLI | 24 | Transcriptional regulatory protein RtcR OS=Escherichia coli (strain K12) GN=rtcR PE=4 SV=2 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADA_ECOLI | 24 | Bifunctional transcriptional activator/DNA repair enzyme Ada OS=Escherichia coli (strain K12) GN=ada PE=1 SV=2 |
Not what you expected? Try the select summary.
Mascot: http://www.matrixscience.com/