MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 38)

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+11

Accession Score Description
1 SAPF_ECOLI 29 Peptide transport system ATP-binding protein SapF OS=Escherichia coli (strain K12) GN=sapF PE=1 SV=1

+12

Accession Score Description
1 DAMX_ECOLI 28 Protein DamX OS=Escherichia coli (strain K12) GN=damX PE=1 SV=2

+13

Accession Score Description
1 YNJD_ECOLI 27 Uncharacterized ABC transporter ATP-binding protein YnjD OS=Escherichia coli (strain K12) GN=ynjD PE=3 SV=2

+14

Accession Score Description
1 ZUR_ECOLI 26 Zinc uptake regulation protein OS=Escherichia coli (strain K12) GN=zur PE=1 SV=1

-15

Accession Score Description
1 VIOA_CHRVO 26 Probable L-tryptophan oxidase VioA OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) GN=vioA PE=1 SV=2
Score Mass Matches Sequences emPAI
15.1 VIOA_CHRVO 26 47118 9 (3) 2 (1) 0.15
Probable L-tryptophan oxidase VioA OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) GN=vioA PE=1 SV=2
1 sameset of VIOA_CHRVO
YEDV_ECOLI 26 0 8 (3) 1 (1) 0.14
description

-9 peptide matches (3 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2516 +1 642.4322 641.4249 641.4224 3.90 0 19 0.017 +4Score > 14 indicates identity U R.LLLQR.I
2517 +5 321.7205 641.4264 641.4224 6.27 0 19 0.015 +1Score > 14 indicates identity U R.LLLQR.I
2789   308.8495 923.5267 923.5116 16.3 0 1 1.1 +1Score > 14 indicates identity U R.YGSLPLFK.G

+16

Accession Score Description
1 CMOB_ECOLI 25 tRNA (mo5U34)-methyltransferase OS=Escherichia coli (strain K12) GN=cmoB PE=3 SV=1

+17

Accession Score Description
1 ODP2_ECOLI 25 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3

+18

Accession Score Description
1 TOP3_ECOLI 25 DNA topoisomerase 3 OS=Escherichia coli (strain K12) GN=topB PE=1 SV=1

+19

Accession Score Description
1 RTCR_ECOLI 24 Transcriptional regulatory protein RtcR OS=Escherichia coli (strain K12) GN=rtcR PE=4 SV=2

+20

Accession Score Description
1 ADA_ECOLI 24 Bifunctional transcriptional activator/DNA repair enzyme Ada OS=Escherichia coli (strain K12) GN=ada PE=1 SV=2
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