MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1249__1.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,429 residues)
Timestamp : 23 Jun 2025 at 17:07:31 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,592

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (40 proteins)

Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1 Q9EX54_STRCO 287 42499 19 (18) 5 (5) 0.82 Putative type I polyketide synthase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO6273 PE=4 SV=1
2.1 K2C1_HUMAN 196 66170 12 (7) 12 (7) 0.40 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6
2.2 K22E_HUMAN 114 65678 8 (6) 8 (6) 0.34 Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2
3 TRYP_PIG 188 25078 7 (5) 4 (4) 0.65 Trypsin OS=Sus scrofa PE=1 SV=1
4 EFTU1_ECOLI 168 43427 9 (6) 2 (1) 0.16 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1
5.1 K1C10_HUMAN 145 59020 16 (9) 15 (9) 0.63 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
5.2 K1C9_HUMAN 101 62255 12 (7) 6 (6) 0.36 Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3
6 RS1_ECOLI 97 61235 9 (5) 4 (3) 0.23 30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1
7 RL4_ECOLI 65 22073 1 (1) 1 (1) 0.15 50S ribosomal protein L4 OS=Escherichia coli (strain K12) GN=rplD PE=1 SV=1
8 ISPA_ECOLI 58 32368 5 (3) 1 (1) 0.10 Farnesyl diphosphate synthase OS=Escherichia coli (strain K12) GN=ispA PE=1 SV=1
9 GLPB_ECOLI 44 45899 2 (2) 1 (1) 0.07 Anaerobic glycerol-3-phosphate dehydrogenase subunit B OS=Escherichia coli (strain K12) GN=glpB PE=1 SV=1
10 RL7_ECOLI 42 12288 1 (1) 1 (1) 0.28 50S ribosomal protein L7/L12 OS=Escherichia coli (strain K12) GN=rplL PE=1 SV=2
11 SAPF_ECOLI 29 30551 3 (1) 2 (1) 0.11 Peptide transport system ATP-binding protein SapF OS=Escherichia coli (strain K12) GN=sapF PE=1 SV=1
12 DAMX_ECOLI 28 46134 1 (1) 1 (1) 0.07 Protein DamX OS=Escherichia coli (strain K12) GN=damX PE=1 SV=2
13 YNJD_ECOLI 27 24173 3 (2) 1 (1) 0.30 Uncharacterized ABC transporter ATP-binding protein YnjD OS=Escherichia coli (strain K12) GN=ynjD PE=3 SV=2
14 ZUR_ECOLI 26 19755 4 (1) 1 (1) 0.17 Zinc uptake regulation protein OS=Escherichia coli (strain K12) GN=zur PE=1 SV=1
15 VIOA_CHRVO 26 47118 9 (3) 2 (1) 0.15 Probable L-tryptophan oxidase VioA OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) GN=vioA PE=1 SV=2
16 CMOB_ECOLI 25 37154 6 (1) 2 (1) 0.09 tRNA (mo5U34)-methyltransferase OS=Escherichia coli (strain K12) GN=cmoB PE=3 SV=1
17 ODP2_ECOLI 25 66112 2 (1) 1 (1) 0.05 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3
18 TOP3_ECOLI 25 73855 3 (2) 2 (1) 0.04 DNA topoisomerase 3 OS=Escherichia coli (strain K12) GN=topB PE=1 SV=1
19 RTCR_ECOLI 24 60604 1 (1) 1 (1) 0.05 Transcriptional regulatory protein RtcR OS=Escherichia coli (strain K12) GN=rtcR PE=4 SV=2
20 ADA_ECOLI 24 39983 3 (1) 2 (1) 0.08 Bifunctional transcriptional activator/DNA repair enzyme Ada OS=Escherichia coli (strain K12) GN=ada PE=1 SV=2
21 FABB_ECOLI 22 42928 2 (1) 2 (1) 0.08 3-oxoacyl-[acyl-carrier-protein] synthase 1 OS=Escherichia coli (strain K12) GN=fabB PE=1 SV=1
22 GSH1_ECOLI 22 58745 7 (1) 1 (1) 0.06 Glutamate--cysteine ligase OS=Escherichia coli (strain K12) GN=gshA PE=1 SV=1
23 NARQ_ECOLI 21 0 5 (2) 1 (1) 0.11 description
24 YHII_ECOLI 20 38805 3 (1) 3 (1) 0.09 Uncharacterized protein YhiI OS=Escherichia coli (strain K12) GN=yhiI PE=4 SV=1
25 FLIG_ECOLI 20 36753 1 (1) 1 (1) 0.09 Flagellar motor switch protein FliG OS=Escherichia coli (strain K12) GN=fliG PE=1 SV=1
26 MOAE_ECOLI 20 0 4 (1) 1 (1) 0.20 description
27 RSMA_ECOLI 20 30515 5 (1) 1 (1) 0.11 Ribosomal RNA small subunit methyltransferase A OS=Escherichia coli (strain K12) GN=rsmA PE=1 SV=1
28 YBIP_ECOLI 20 0 4 (1) 1 (1) 0.06 description
29 YNIA_ECOLI 19 32666 4 (1) 2 (1) 0.10 Uncharacterized protein YniA OS=Escherichia coli (strain K12) GN=yniA PE=3 SV=1
30 GNTR_ECOLI 18 36570 1 (1) 1 (1) 0.09 HTH-type transcriptional regulator GntR OS=Escherichia coli (strain K12) GN=gntR PE=1 SV=1
31 NFRA_ECOLI 18 111468 5 (1) 3 (1) 0.03 Bacteriophage N4 adsorption protein A OS=Escherichia coli (strain K12) GN=nfrA PE=4 SV=1
32 ADEC_ECOLI 18 64326 1 (1) 1 (1) 0.05 Adenine deaminase OS=Escherichia coli (strain K12) GN=ade PE=1 SV=2
33 Q1XAB0_CHOFU 18 51396 2 (1) 2 (1) 0.06 Putative farnesyl diphosphate synthase OS=Choristoneura fumiferana PE=2 SV=1
34 PGAC_ECOLI 16 51303 1 (1) 1 (1) 0.06 Poly-beta-1,6-N-acetyl-D-glucosamine synthase OS=Escherichia coli (strain K12) GN=pgaC PE=1 SV=1
35 A4U8R3_9BACT 16 362145 6 (1) 5 (1) 0.01 SupA OS=Aplysina aerophoba bacterial symbiont clone pAPKS18 GN=supA PE=4 SV=1
36 PSUT_ECOLI 15 0 1 (1) 1 (1) 0.07 description
37 CH60_ECOLI 14 57464 1 (1) 1 (1) 0.06 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2
38 HLYE_ECOLI 13 33852 2 (1) 1 (1) 0.10 Hemolysin E, chromosomal OS=Escherichia coli (strain K12) GN=hlyE PE=1 SV=4

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