MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
268 309.1691 308.1618
269 309.1691 308.1618
270 309.1691 308.1618
271 309.1691 308.1618
272 309.1692 308.1619
273 309.1692 308.1619
274 309.1692 308.1619
275 309.1695 308.1622
276 309.1876 308.1803
277 309.1888 308.1815
278 309.1891 308.1818
279 309.2057 308.1984
280 309.2061 308.1988
281 309.2061 308.1988
282 309.2062 308.1989
283 309.2064 308.1991
284 309.2070 308.1997
285 309.2077 308.2004
286 310.0150 309.0077
287 310.5335 309.5262
288 311.0744 310.0671
289 311.0812 310.0739
290 311.0814 310.0741
291 311.0816 310.0743
292 311.0818 310.0745
293 311.0818 310.0745
294 311.0822 310.0749
295 311.0826 310.0753
296 311.0907 310.0834
297 311.0909 310.0836
298 311.0919 310.0846
299 311.1001 310.0928
300 311.1111 310.1038
301 311.1112 310.1039
302 311.1112 310.1039
303 311.1113 310.1040
304 311.1115 310.1042
305 311.1115 310.1042
306 311.1115 310.1042
307 311.1116 310.1043
308 311.1117 310.1044
309 311.1123 310.1050
310 311.1127 310.1054
311 311.1476 310.1403
312 311.1479 310.1406
313 311.1480 310.1407
314 311.1480 310.1407
315 311.1480 310.1407
316 311.1481 310.1408
317 311.1481 310.1408
318 311.1482 310.1409
319 311.1482 310.1409
320 311.1482 310.1409
321 311.1483 310.1410
322 311.1483 310.1410
323 311.1484 310.1411
324 311.1484 310.1411
325 311.1485 310.1412
326 311.1487 310.1414
327 311.1487 310.1414
328 311.1495 310.1422
329 311.1660 310.1587
330 311.1660 310.1587
331 311.1662 310.1589
332 311.1662 310.1589
333 311.1666 310.1593
334 311.1667 310.1594
335 311.1843 310.1770
336 311.1846 310.1773
337 311.1846 310.1773
338 311.1848 310.1775
339 311.1848 310.1775
340 311.1848 310.1775
341 311.2227 310.2154
342 311.2228 310.2155
343 311.2231 310.2158
344 312.0306 311.0233
345 312.1318 311.1245
346 312.1319 311.1246
347 312.1322 311.1249
348 312.1519 311.1446
349 312.2076 311.2003
350 313.0222 312.0149
351 313.0299 312.0226
352 313.0602 312.0529
353 313.0608 312.0535
354 313.0609 312.0536
355 313.0611 312.0538
356 313.0612 312.0539
357 313.0890 312.0817
358 313.0891 312.0818
359 313.0968 312.0895
360 313.0978 312.0905
361 313.0999 312.0926
362 313.1158 312.1085
363 313.1159 312.1086
364 313.1169 312.1096
365 313.1269 312.1196
366 313.1271 312.1198
367 313.1277 312.1204
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