MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
168 307.1519 306.1446
169 307.1528 306.1455
170 307.1529 306.1456
171 307.1530 306.1457
172 307.1530 306.1457
173 307.1530 306.1457
174 307.1530 306.1457
175 307.1531 306.1458
176 307.1531 306.1458
177 307.1532 306.1459
178 307.1532 306.1459
179 307.1532 306.1459
180 307.1533 306.1460
181 307.1533 306.1460
182 307.1534 306.1461
183 307.1534 306.1461
184 307.1534 306.1461
185 307.1534 306.1461
186 307.1534 306.1461
187 307.1535 306.1462
188 307.1537 306.1464
189 307.1897 306.1824
190 307.1897 306.1824
191 307.1898 306.1825
192 307.1899 306.1826
193 307.1899 306.1826
194 307.1899 306.1826
195 307.1900 306.1827
196 307.1900 306.1827
197 307.1900 306.1827
198 307.1901 306.1828
199 307.1902 306.1829
200 307.1902 306.1829
201 307.1902 306.1829
202 307.1903 306.1830
203 307.1903 306.1830
204 307.1903 306.1830
205 307.1904 306.1831
206 307.2099 306.2026
207 307.9947 306.9874
208 307.9947 306.9874
209 308.1570 307.1497
210 309.0569 308.0496
211 309.0663 308.0590
212 309.0669 308.0596
213 309.0937 308.0864
214 309.0945 308.0872
215 309.0945 308.0872
216 309.0953 308.0880
217 309.0953 308.0880
218 309.0955 308.0882
219 309.0955 308.0882
220 309.0958 308.0885
221 309.0961 308.0888
222 309.0974 308.0901
223 309.1309 308.1236
224 309.1313 308.1240
225 309.1317 308.1244
226 309.1317 308.1244
227 309.1318 308.1245
228 309.1321 308.1248
229 309.1322 308.1249
230 309.1323 308.1250
231 309.1324 308.1251
232 309.1324 308.1251
233 309.1324 308.1251
234 309.1324 308.1251
235 309.1325 308.1252
236 309.1325 308.1252
237 309.1325 308.1252
238 309.1326 308.1253
239 309.1327 308.1254
240 309.1327 308.1254
241 309.1327 308.1254
242 309.1327 308.1254
243 309.1328 308.1255
244 309.1328 308.1255
245 309.1382 308.1309
246 309.1383 308.1310
247 309.1384 308.1311
248 309.1384 308.1311
249 309.1388 308.1315
250 309.1511 308.1438
251 309.1511 308.1438
252 309.1687 308.1614
253 309.1688 308.1615
254 309.1688 308.1615
255 309.1688 308.1615
256 309.1688 308.1615
257 309.1689 308.1616
258 309.1689 308.1616
259 309.1689 308.1616
260 309.1689 308.1616
261 309.1689 308.1616
262 309.1690 308.1617
263 309.1690 308.1617
264 309.1690 308.1617
265 309.1690 308.1617
266 309.1690 308.1617
267 309.1690 308.1617
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