MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
68 303.1580 302.1507
69 303.1580 302.1507
70 303.1580 302.1507
71 303.1581 302.1508
72 303.1581 302.1508
73 303.1582 302.1509
74 303.1582 302.1509
75 303.1582 302.1509
76 303.1583 302.1510
77 303.1585 302.1512
78 304.0665 303.0592
79 304.1656 303.1583
80 305.0628 304.0555
81 305.0989 304.0916
82 305.0989 304.0916
83 305.0989 304.0916
84 305.0993 304.0920
85 305.0995 304.0922
86 305.1000 304.0927
87 305.1000 304.0927
88 305.1007 304.0934
89 305.1008 304.0935
90 305.1008 304.0935
91 305.1009 304.0936
92 305.1009 304.0936
93 305.1011 304.0938
94 305.1012 304.0939
95 305.1020 304.0947
96 305.1369 304.1296
97 305.1371 304.1298
98 305.1371 304.1298
99 305.1371 304.1298
100 305.1373 304.1300
101 305.1374 304.1301
102 305.1374 304.1301
103 305.1374 304.1301
104 305.1375 304.1302
105 305.1375 304.1302
106 305.1375 304.1302
107 305.1375 304.1302
108 305.1376 304.1303
109 305.1376 304.1303
110 305.1376 304.1303
111 305.1376 304.1303
112 305.1376 304.1303
113 305.1376 304.1303
114 305.1376 304.1303
115 305.1377 304.1304
116 305.1377 304.1304
117 305.1377 304.1304
118 305.1377 304.1304
119 305.1587 304.1514
120 305.1587 304.1514
121 305.1737 304.1664
122 305.1737 304.1664
123 305.1738 304.1665
124 305.1738 304.1665
125 305.1738 304.1665
126 305.1738 304.1665
127 305.1739 304.1666
128 305.1739 304.1666
129 305.1740 304.1667
130 305.1740 304.1667
131 305.1740 304.1667
132 305.1741 304.1668
133 305.1741 304.1668
134 305.1743 304.1670
135 305.1743 304.1670
136 305.1743 304.1670
137 305.1744 304.1671
138 305.1748 304.1675
139 305.1749 304.1676
140 305.2104 304.2031
141 306.1609 305.1536
142 306.1612 305.1539
143 307.0103 306.0030
144 307.0118 306.0045
145 307.0210 306.0137
146 307.0785 306.0712
147 307.0785 306.0712
148 307.0789 306.0716
149 307.0797 306.0724
150 307.1151 306.1078
151 307.1155 306.1082
152 307.1156 306.1083
153 307.1159 306.1086
154 307.1162 306.1089
155 307.1163 306.1090
156 307.1164 306.1091
157 307.1164 306.1091
158 307.1164 306.1091
159 307.1166 306.1093
160 307.1167 306.1094
161 307.1167 306.1094
162 307.1167 306.1094
163 307.1168 306.1095
164 307.1168 306.1095
165 307.1169 306.1096
166 307.1169 306.1096
167 307.1169 306.1096
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