MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4247)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3778 501.7983 1001.5820 1001.5692 12.9 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
KMIQLVDR
4410 823.1196 2466.3370 2466.2894 19.3 0 1 4 +1Score > 19 indicates identity QNLLYLVDTYPVTIVVGQTGSGK + 2 Deamidated (NQ)
4250 554.9328 1661.7766 1661.7856 -5.44 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
TQESQNSEPASKSIR + Deamidated (NQ)
3942 394.5109 1180.5109 1180.4964 12.2 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
RNMQNEMNK + Deamidated (NQ); Oxidation (M)
4393 786.4066 2356.1980 2356.2063 -3.53 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
ALVDAALNNNVKFFVYTSVDR + Deamidated (NQ)
3924 578.3260 1154.6374 1154.6408 -2.87 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
KPSTGGKAPAGGK
4396 796.4301 2386.2685 2386.2910 -9.42 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
HWRLIGWANIVAGNTLEPILN
3473 344.1948 686.3750 686.3711 5.74 0 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology
LAQEAR
3986 619.8746 1237.7346 1237.7394 -3.84 0 0 1.7 +1Score > 15 indicates identity AIGAVNTLIPIR + Deamidated (NQ)
4307 620.9955 1859.9647 1859.9411 12.7 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
SLGNDDMVIKAQVLAGGR + Deamidated (NQ); Oxidation (M)
4446 835.8918 3339.5381 3339.5097 8.49 1 0 0.91 +1Score > 23 indicates identity
Score > 13 indicates homology
LEYVWIDTCCIDKSSSAELSESINSMFR
3714 465.7586 929.5026 929.4970 6.05 1 0 1 +1Score > 24 indicates identity
Score > 13 indicates homology
KPKWQNK + 2 Deamidated (NQ)
3813 344.8679 1031.5819 1031.5797 2.08 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NVMIVTKAR + Deamidated (NQ)
4089 455.5901 1363.7485 1363.7684 -14.6 1 0 0.95 +1Score > 20 indicates identity
Score > 13 indicates homology
ELNVHAVLTGRR
4123 705.9235 1409.8324 1409.8143 12.9 1 0 1.7 +1Score > 15 indicates identity NKVALIPNSLWR
4400 597.8058 2387.1941 2387.2260 -13.4 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
VLDVLAVVDKTPIGDFWNEQK + 2 Deamidated (NQ)
4141 481.9153 1442.7241 1442.7374 -9.22 1 0 0.94 +1Score > 23 indicates identity
Score > 13 indicates homology
GLGDIKNMVPMPR + Oxidation (M)
3964 602.3259 1202.6372 1202.6216 13.0 0 0 1 +1Score > 24 indicates identity
Score > 13 indicates homology
SALPLVQQMSK + 2 Deamidated (NQ)
4326 973.5108 1945.0070 1945.0078 -0.37 0 0 0.97 +1Score > 23 indicates identity
Score > 13 indicates homology
DMSALVELIIELQDQTK
4336 659.3507 1975.0303 1975.0123 9.12 1 0 0.98 +1Score > 23 indicates identity
Score > 13 indicates homology
SKPLADSVAASLGADRFNR + Deamidated (NQ)
3688 456.7875 911.5604 911.5440 18.1 0 0 2.6 +1Score > 17 indicates identity LLTEALPR
3629 428.2527 854.4908 854.4861 5.50 0 0 3.2 +1Score > 18 indicates identity EVEPLLR
3814 519.2606 1036.5066 1036.5189 -11.8 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
VFSSIDVNR + Deamidated (NQ)
3948 595.3403 1188.6660 1188.6767 -9.00 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology
NFVKHILYR
4137 477.2652 1428.7738 1428.7612 8.77 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
DQWKQLADLALK + Deamidated (NQ)
4151 367.1752 1464.6717 1464.6588 8.78 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
GSVLNKMNNDPMK + 2 Deamidated (NQ); Oxidation (M)
4267 572.3320 1713.9742 1714.0029 -16.8 0 0 3 +1Score > 17 indicates identity QILITGAAGFIGQLLAK + Deamidated (NQ)
4104 458.2358 1371.6856 1371.7068 -15.5 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
SQLCTKAQLPPK + 2 Deamidated (NQ)
3838 528.2465 1054.4784 1054.4713 6.78 1 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
KTAGNSSSMR + Deamidated (NQ); Oxidation (M)
4380 563.2721 2249.0593 2249.0647 -2.41 1 0 1 +1Score > 24 indicates identity
Score > 13 indicates homology
HTAHDMANLQEYPNPKGAQK
3996 625.8257 1249.6368 1249.6302 5.29 0 0 1 +1Score > 24 indicates identity
Score > 13 indicates homology
DNLAPLEPEPR
4272 863.9123 1725.8100 1725.7848 14.6 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
TCGMQVATELALTCR + Oxidation (M)
4345 683.6913 2048.0521 2048.0650 -6.34 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
AEATLHNAPIVIDNGSGTIR
1 300.0849 299.0776
2 300.1515 299.1442
3 300.1820 299.1747
4 300.1822 299.1749
5 301.0600 300.0527
6 301.0608 300.0535
7 301.0880 300.0807
8 301.0981 300.0908
9 301.1053 300.0980
10 301.1053 300.0980
11 301.1055 300.0982
12 301.1055 300.0982
13 301.1055 300.0982
14 301.1060 300.0987
15 301.1236 300.1163
16 301.1291 300.1218
17 301.1297 300.1224
18 301.1419 300.1346
19 301.1420 300.1347
20 301.1422 300.1349
21 301.1422 300.1349
22 301.1423 300.1350
23 301.1423 300.1350
24 301.1423 300.1350
25 301.1424 300.1351
26 301.1424 300.1351
27 301.1424 300.1351
28 301.1424 300.1351
29 301.1425 300.1352
30 301.1425 300.1352
31 301.1425 300.1352
32 301.1425 300.1352
33 301.1426 300.1353
34 301.1427 300.1354
35 301.1512 300.1439
36 301.2206 300.2133
37 302.1031 301.0958
38 302.1978 301.1905
39 302.1978 301.1905
40 302.1980 301.1907
41 303.0392 302.0319
42 303.0747 302.0674
43 303.0749 302.0676
44 303.0762 302.0689
45 303.0838 302.0765
46 303.0839 302.0766
47 303.0841 302.0768
48 303.0847 302.0774
49 303.0847 302.0774
50 303.0851 302.0778
51 303.0852 302.0779
52 303.1034 302.0961
53 303.1213 302.1140
54 303.1214 302.1141
55 303.1215 302.1142
56 303.1216 302.1143
57 303.1216 302.1143
58 303.1216 302.1143
59 303.1216 302.1143
60 303.1216 302.1143
61 303.1217 302.1144
62 303.1218 302.1145
63 303.1218 302.1145
64 303.1218 302.1145
65 303.1219 302.1146
66 303.1220 302.1147
67 303.1579 302.1506
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