MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3685 303.1797 906.5173 906.5035 15.1 1 2 2.6 +1Score > 19 indicates identity NVYRTVR
3889 558.3220 1114.6294 1114.6346 -4.61 1 2 0.89 +1Score > 23 indicates identity
Score > 14 indicates homology		 LNTREIIEK
4305 928.5013 1854.9880 1854.9938 -3.10 1 2 0.92 +1Score > 22 indicates identity
Score > 14 indicates homology		 DLKPENLLIDSEKNVK + Deamidated (NQ)
3864 544.2941 1086.5736 1086.5934 -18.2 1 2 1 +1Score > 24 indicates identity
Score > 14 indicates homology		 FLGPNNLRR + Deamidated (NQ)
4175 753.9160 1505.8174 1505.7950 14.9 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 AAGGENPHTRLAALK + Deamidated (NQ)
3819 522.7966 1043.5786 1043.5723 6.06 1 2 1 +1Score > 24 indicates identity
Score > 14 indicates homology		 KSAPSASQLR
4005 631.3532 1260.6918 1260.6727 15.2 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 LRNAWFNIAR + Deamidated (NQ)
4042 654.3523 1306.6900 1306.6703 15.1 0 2 0.95 +1Score > 23 indicates identity
Score > 14 indicates homology		 INGLFIESMQR
4292 602.6658 1804.9756 1804.9427 18.2 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 LAPSQLVMIRDMISSK + Deamidated (NQ); Oxidation (M)
3931 581.3061 1160.5976 1160.5860 10.1 1 2 1 +1Score > 24 indicates identity
Score > 14 indicates homology		 DVEDIMGRVK
3712 465.2684 928.5222 928.5229 -0.72 0 2 0.69 +1Score > 24 indicates identity
Score > 13 indicates homology		 QIGLEIQK + Deamidated (NQ)
3510 364.2132 726.4118 726.4137 -2.48 0 2 3 +1Score > 19 indicates identity LGRPER
3854 360.8279 1079.4619 1079.4441 16.5 0 2 3.2 +1Score > 19 indicates identity VSESCSPSEV
4398 597.5751 2386.2713 2386.2467 10.3 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 WPLQSPPMERALHAVSEVVPK + Oxidation (M)
4131 476.5833 1426.7281 1426.7304 -1.61 0 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 EGLDITLSDIHSK
4258 846.4533 1690.8920 1690.9042 -7.20 1 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 EYLPAFAVREDLLR
3751 973.5394 972.5321 972.5141 18.5 1 2 1 +1Score > 25 indicates identity
Score > 14 indicates homology		 GAWLNEKR
4090 455.5901 1363.7485 1363.7711 -16.6 1 2 0.79 +1Score > 20 indicates identity
Score > 13 indicates homology		 TGALYANKTLLAK + Deamidated (NQ)
4088 455.5900 1363.7482 1363.7459 1.64 1 2 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 FSTLIRTLNGNK + Deamidated (NQ)
3574 401.2297 800.4448 800.4504 -6.95 1 2 0.78 +1Score > 24 indicates identity
Score > 13 indicates homology		 LAKANQR + Deamidated (NQ)
4107 689.8921 1377.7696 1377.7517 13.0 1 2 0.81 +1Score > 20 indicates identity
Score > 13 indicates homology		 FQAFGRPVKNSK
3777 334.8678 1001.5816 1001.5757 5.88 1 2 0.96 +1Score > 21 indicates identity
Score > 14 indicates homology		 KTSSIPELK
4187 768.9080 1535.8014 1535.7831 11.9 0 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 TLVHEDNPPVTSVK + Deamidated (NQ)
3628 426.7213 851.4280 851.4389 -12.7 1 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 SVKFQDK + Deamidated (NQ)
4340 668.0335 2001.0787 2001.1146 -18.0 1 2 0.99 +1Score > 21 indicates identity
Score > 14 indicates homology		 ESALQLLGPPKYATTLATK
3805 342.8243 1025.4511 1025.4713 -19.7 1 2 3.5 +1Score > 19 indicates identity SMDRFSQR
4189 770.9424 1539.8702 1539.8620 5.36 1 2 3.1 +1Score > 19 indicates identity IAIVQELAENANKK
3803 513.3426 1024.6706 1024.6644 6.05 0 2 0.71 +1Score > 13 indicates identity AIQIAGLVIK
3987 621.8406 1241.6666 1241.6516 12.1 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 ISQAINRPWR + 2 Deamidated (NQ)
3669 446.7282 891.4418 891.4371 5.27 0 1 0.78 +1Score > 24 indicates identity
Score > 13 indicates homology		 ELGMIDAK + Oxidation (M)
3734 475.7589 949.5032 949.4869 17.2 0 1 0.95 +1Score > 23 indicates identity
Score > 14 indicates homology		 VEEAFLSR
3941 394.2095 1179.6067 1179.5996 5.97 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 RSSFGSSPSIR
4039 435.5160 1303.5262 1303.5173 6.84 0 1 1.3 +1Score > 15 indicates identity HEEMQVMEDR + Deamidated (NQ)
3836 527.7667 1053.5188 1053.5203 -1.40 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 HTTPASPSTR
4417 647.5665 2586.2369 2586.1874 19.1 0 1 0.98 +1Score > 24 indicates identity
Score > 14 indicates homology		 SIDHNQYVIDGLTYQANYGSGLR + 3 Deamidated (NQ)
4138 718.3732 1434.7318 1434.7466 -10.3 1 1 0.89 +1Score > 23 indicates identity
Score > 13 indicates homology		 NFIISQLESARR + 2 Deamidated (NQ)
4291 602.6656 1804.9750 1804.9427 17.9 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 LAPSQLVMIRDMISSK + Deamidated (NQ); Oxidation (M)
3666 445.7611 889.5076 889.5021 6.20 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 GIPITYAR
4315 629.6688 1885.9846 1885.9687 8.44 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 RLQGFGVDWQDLPQVK + Deamidated (NQ)
3869 364.5388 1090.5946 1090.6135 -17.4 1 1 0.93 +1Score > 23 indicates identity
Score > 14 indicates homology		 KTGQGNVFIK
3990 414.9212 1241.7418 1241.7244 14.0 1 1 1.7 +1Score > 16 indicates identity WVLRSDLVVR
3719 312.5167 934.5283 934.5097 19.9 1 1 0.93 +1Score > 21 indicates identity
Score > 13 indicates homology		 RAGIPEHR
4289 901.9488 1801.8830 1801.8821 0.53 1 1 1 +1Score > 24 indicates identity
Score > 14 indicates homology		 QEIYYALAMQRFPR + Deamidated (NQ); Oxidation (M)
3808 514.8116 1027.6086 1027.6026 5.91 0 1 0.88 +1Score > 21 indicates identity
Score > 13 indicates homology		 VGIALESALR
3672 447.7403 893.4660 893.4607 6.03 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 ISQDLYR
3782 502.7955 1003.5764 1003.5662 10.2 1 1 3.4 +1Score > 19 indicates identity KSGETITIR
4130 714.3712 1426.7278 1426.7092 13.0 0 1 0.82 +1Score > 23 indicates identity
Score > 13 indicates homology		 IGPESPNFVDVPR + Deamidated (NQ)
3837 527.8331 1053.6516 1053.6447 6.58 1 1 1.1 +1Score > 14 indicates identity RQFLLHIK
4140 480.9471 1439.8195 1439.7919 19.2 1 1 3.3 +1Score > 19 indicates identity QACKAILSVPTPR
3462 673.4282 672.4209 672.4170 5.80 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 VSIIGGK
3876 550.3092 1098.6038 1098.6033 0.48 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 VQATVSPAAVR + Deamidated (NQ)
4111 693.8997 1385.7848 1385.7878 -2.14 1 1 3.8 +1Score > 19 indicates identity LQQVVKVSESLR + Deamidated (NQ)
3893 560.7944 1119.5742 1119.5957 -19.2 1 1 0.99 +1Score > 24 indicates identity
Score > 14 indicates homology		 KAAEMLNSIK + Oxidation (M)
4002 628.3647 1254.7148 1254.6932 17.3 0 1 0.9 +1Score > 20 indicates identity
Score > 13 indicates homology		 ALTASPTPIAASR
3717 311.5028 931.4866 931.4909 -4.65 0 1 0.95 +1Score > 25 indicates identity
Score > 13 indicates homology		 TAIAAMAQR
4062 663.9072 1325.7998 1325.7779 16.6 1 1 1 +1Score > 14 indicates identity ALLETLNAGRIR
4006 421.5738 1261.6996 1261.7084 -6.99 1 1 0.95 +1Score > 21 indicates identity
Score > 13 indicates homology		 RLWWSIFVR
4023 641.8158 1281.6170 1281.6313 -11.1 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 TETYQQVTRR + Deamidated (NQ)
4181 505.9417 1514.8033 1514.7940 6.11 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology		 VGSELLQESAQVVR + Deamidated (NQ)
4399 597.5752 2386.2717 2386.2467 10.5 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 WPLQSPPMERALHAVSEVVPK + Oxidation (M)
3872 365.5267 1093.5583 1093.5516 6.10 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 RVLDNSSFR + Deamidated (NQ)
4128 712.8175 1423.6204 1423.6063 9.95 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 QTQNTNGSQTNTK + 3 Deamidated (NQ)
4188 513.2670 1536.7792 1536.7644 9.59 1 1 0.98 +1Score > 23 indicates identity
Score > 13 indicates homology		 NDNVLNQPNPQRK + Deamidated (NQ)
3398 314.7249 627.4352 627.4319 5.26 0 1 1.9 +1Score > 16 indicates identity VLGVIK
3686 454.7665 907.5184 907.5239 -6.04 1 1 3.4 +1Score > 19 indicates identity LTRSYLR
4001 628.3061 1254.5976 1254.5880 7.66 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology		 QFEIDNYLGR + Deamidated (NQ)
4079 455.2512 1362.7318 1362.7190 9.37 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology		 MAIPSKGPAQHAR
4177 757.4136 1512.8126 1512.8201 -4.93 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 KWGIEQLLANWR
4271 863.4331 1724.8516 1724.8217 17.4 1 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology		 SLKVYEQGISDSENR + Deamidated (NQ)
4227 803.4211 1604.8276 1604.8369 -5.79 1 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology		 LAKTSGELNGVSTSNK
4037 651.8600 1301.7054 1301.7013 3.19 1 1 0.9 +1Score > 23 indicates identity
Score > 13 indicates homology		 MSGVKPAEVKEK
4161 742.3666 1482.7186 1482.7024 10.9 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology		 SENFDKAVEVMAK + Oxidation (M)
3576 401.7212 801.4278 801.4344 -8.24 1 1 0.87 +1Score > 25 indicates identity
Score > 13 indicates homology		 TAQTPKR + Deamidated (NQ)
3810 515.7509 1029.4872 1029.4992 -11.6 0 1 0.85 +1Score > 21 indicates identity
Score > 13 indicates homology		 QLAEHYNR
3839 352.8705 1055.5897 1055.5764 12.6 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology		 VVPQFPNVR + Deamidated (NQ)
3999 418.2368 1251.6886 1251.6935 -3.96 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 LASTIGIAVDHR
4449 864.1753 3452.6721 3452.6524 5.70 1 1 0.88 +1Score > 23 indicates identity
Score > 13 indicates homology		 WELLQQVDTSTRTHNLEPYFESFINNLR + 3 Deamidated (NQ)
3852 539.3045 1076.5944 1076.5760 17.1 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 MVALRTSAGR + Oxidation (M)
3978 408.5616 1222.6630 1222.6856 -18.5 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 HVQLPKNMLK + Oxidation (M)
3625 425.7448 849.4750 849.4742 0.98 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology		 VKSMTIR + Oxidation (M)
3882 370.1948 1107.5626 1107.5561 5.88 0 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology		 TTAFVGPSGSGK
3967 607.3411 1212.6676 1212.6575 8.39 1 1 0.95 +1Score > 22 indicates identity
Score > 13 indicates homology		 EVIRVQGAGAGR + Deamidated (NQ)
4086 455.5899 1363.7479 1363.7646 -12.3 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 NKVLVFQLMTR + Oxidation (M)
4156 737.3942 1472.7738 1472.7769 -2.08 1 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology		 ALGSSMHSKASLIR + Oxidation (M)
3849 537.2311 1072.4476 1072.4509 -2.99 0 1 2.2 +1Score > 17 indicates identity AGQMGYHHR + Deamidated (NQ); Oxidation (M)
4427 904.7955 2711.3647 2711.3548 3.64 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology		 GADVNALPRGMTALQAAVASNSPEVVR + 2 Deamidated (NQ); Oxidation (M)
3976 611.7934 1221.5722 1221.5659 5.18 0 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology		 NSVNMLQTSAR + 2 Deamidated (NQ)
4413 836.1246 2505.3520 2505.3380 5.59 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 YLSWVINETLRLYPVVPSNSR
4432 910.7906 2729.3500 2729.3337 5.96 0 1 0.99 +1Score > 24 indicates identity
Score > 13 indicates homology		 HFGALFLNFYSPITTGNITSTSSPR + 2 Deamidated (NQ)
4054 660.8528 1319.6910 1319.6755 11.8 1 1 0.89 +1Score > 23 indicates identity
Score > 13 indicates homology		 LTKESNICDLK
4103 458.2351 1371.6835 1371.7068 -17.0 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology		 SQLCTKAQLPPK + 2 Deamidated (NQ)
4395 796.4295 2386.2667 2386.2910 -10.2 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 HWRLIGWANIVAGNTLEPILN
4300 618.0095 1851.0067 1850.9812 13.8 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 MYGSLIADNTLNLGILK + Oxidation (M)
4330 653.6460 1957.9162 1957.9091 3.61 1 1 0.94 +1Score > 23 indicates identity
Score > 13 indicates homology		 AAQCLVQSNYTQPGKYK + 3 Deamidated (NQ)
3795 509.2397 1016.4648 1016.4783 -13.2 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 AMHTELMGK
3689 457.2270 912.4394 912.4527 -14.6 0 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 FFEMIAR
4162 743.4294 1484.8442 1484.8463 -1.40 1 1 3.8 +1Score > 19 indicates identity ATIAFGGVLPRINR + Deamidated (NQ)
3776 501.7438 1001.4730 1001.4713 1.79 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology		 MHNLSTQR + Oxidation (M)
4259 567.6338 1699.8796 1699.8716 4.70 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology		 MPTVHLLDYVAGNVR + Oxidation (M)
3566 395.2289 788.4432 788.4578 -18.5 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology		 AVMAIIR + Oxidation (M)
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