MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4142 483.6029 1447.7869
4144 725.4017 1448.7888
4145 485.2564 1452.7474
4146 728.4069 1454.7992
4147 729.3968 1456.7790
4148 730.3795 1458.7444
4149 487.2592 1458.7558
4150 488.5963 1462.7671
4152 489.2328 1464.6766
4153 489.2595 1464.7567
4155 491.2483 1470.7231
4163 496.2469 1485.7189
4167 500.2699 1497.7879
4170 750.4291 1498.8436
4171 751.9355 1501.8564
4176 756.3482 1510.6818
4180 505.9115 1514.7127
4183 758.9449 1515.8752
4190 514.5436 1540.6090
4196 772.4063 1542.7980
4198 777.4346 1552.8546
4199 518.9356 1553.7850
4200 519.2924 1554.8554
4202 523.6224 1567.8454
4203 524.6235 1570.8487
4210 790.9109 1579.8072
4211 791.4124 1580.8102
4212 791.9339 1581.8532
4216 531.9521 1592.8345
4218 532.2682 1593.7828
4226 802.4609 1602.9072
4229 806.3826 1610.7506
4234 808.4197 1614.8248
4235 540.3030 1617.8872
4237 812.4624 1622.9102
4238 541.9783 1622.9131
4239 541.9783 1622.9131
4240 814.3806 1626.7466
4241 409.2227 1632.8617
4242 545.6288 1633.8646
4243 545.6525 1633.9357
4244 546.3292 1635.9658
4248 553.6497 1657.9273
4255 841.4132 1680.8118
4256 564.3110 1689.9112
4257 564.6282 1690.8628
4261 855.4041 1708.7936
4264 857.4918 1712.9690
4265 571.9973 1712.9701
4266 857.4929 1712.9712
4268 572.3320 1713.9742
4269 861.9792 1721.9438
4274 577.6510 1729.9312
4275 871.9321 1741.8496
4276 872.4961 1742.9776
4277 437.1810 1744.6949
4278 437.2487 1744.9657
4279 437.4420 1745.7389
4280 584.9810 1751.9212
4281 878.4863 1754.9580
4282 589.3252 1764.9538
4283 883.4853 1764.9560
4284 884.4464 1766.8782
4293 603.0148 1806.0226
4295 604.6539 1810.9399
4297 610.6194 1828.8364
4298 614.3475 1840.0207
4301 618.0101 1851.0085
4303 464.0098 1852.0101
4309 623.0272 1866.0598
4310 623.0275 1866.0607
4311 938.4440 1874.8734
4312 628.3596 1882.0570
4313 628.6937 1883.0593
4316 631.6939 1892.0599
4318 632.6967 1895.0683
4320 955.8342 1909.6538
4323 641.7011 1922.0815
4324 644.3011 1929.8815
4325 649.0091 1944.0055
4327 977.4633 1952.9120
4328 651.9783 1952.9131
4329 978.5258 1955.0370
4331 653.6778 1958.0116
4333 658.9733 1973.8981
4335 659.3483 1975.0231
4337 989.0215 1976.0284
4338 661.7065 1982.0977
4342 1013.9849 2025.9552
4343 678.7168 2033.1286
4344 1018.0725 2034.1304
4346 513.2734 2049.0645
4347 514.2761 2053.0753
4348 693.3691 2077.0855
4356 707.6865 2120.0377
4357 1061.0278 2120.0410
4358 707.6888 2120.0446
4359 709.0386 2124.0940
4363 540.2546 2156.9893
4366 728.3632 2182.0678
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